grey seal ( Halichoerus grypus )
hgMyo

Western result: +

GST GST-hgMyo (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: hgMyo
CBI Antibody Core Code: A0377
"Lot" Number: 1 Position: 100(1-100)
% Identical Homology of Target to Mouse Protein: 84% (NP_038621.2| myoglobin)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
MGLSDGEWHLVLNVWGKVETDLAGHGQEVLIRLFKSHPET
LEKFDKFKHLKSEDDMRRSEDLRKHGNTVLTALGGILKKK
GHHEAELKPLAQSHATKHKI

Sequence Name: hgMyo
Full name: Myoglobin
UniGene Number: N.A.
Accession Number: V00471
Source organism: grey seal ( Halichoerus grypus )
Full length size (aa): 154
NCBI information page: click here

Full length sequence:
MGLSDGEWHLVLNVWGKVETDLAGHGQEVLIRLFKSHPETLEKFDKFKHL
KSEDDMRRSEDLRKHGNTVLTALGGILKKKGHHEAELKPLAQSHATKHKI
PIKYLEFISEAIIHVLHSKHPAEFGADAQAAMKKALELFRNDIAAKYKEL
GFHG