chicken ( Gallus gallus )
TLP, TRF2

Western result: +

control TLP, TRF2 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: TLP, TRF2
CBI Antibody Core Code: A0597
"Lot" Number: 1 Position: 20(116-135)
% Identical Homology of Target to Mouse Protein: 100% (NP_035733.1| TATA box binding protein-like 1; TBP-related factor 2; TATA box binding protein-like protein; TBP-like protein; TBP-like factor)
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
RLPEFTKNNRPHASYEPELH

Sequence Name: TLP, TRF2
Full name: TATA box binding protein-like protein 1
UniGene Number: N.A.
Accession Number: Q9YGV8
Source organism: chicken ( Gallus gallus )
Full length size (aa): 183
NCBI information page: click here

Full length sequence:
MDADSDVALDILITNVVCVFRTRCHLNLRKIALEGANVIYKRDVGKVLMK
LRKPRITATIWSSGKVICTGATSEEEAKFGARRLARCLQKLGFQVIFTDF
KVVNVLAVCNMPFEIRLPEFTKNNRPHASYEPELHPAVCYRIKTLRATLQ
IFSTGSITVTGPNVKAVASAVEQIYPFVFESRK