chicken ( Gallus gallus )
cTBP

Western result: +/-

GST GST-cTBP (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: cTBP
CBI Antibody Core Code: A0598
"Lot" Number: 2 Position: 100(151-250)
% Identical Homology of Target to Mouse Protein: 100% (NP_038712.1| TATA box binding protein)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
RNAEYNPKRFAAVIMRIREPRTTALIFSSGKMVCTGAKSE
EQSRLAARKYARVVQKLGFPAKFLDFKIQNMVGSCDVKFP
IRLEGLVLTHQQFSSYEPEL

Sequence Name: cTBP
Full name: TBP [Gallus gallus]
UniGene Number: N.A.
Accession Number: BAA20298
Source organism: chicken ( Gallus gallus )
Full length size (aa): 302
NCBI information page: click here

Full length sequence:
MDQNNSLPPYAQGLASPQGAMTPGIPIFSPMMPYGTGLTPQPVQSTNSLS
ILEEQQRQQQQQQAAQSSTSQQATQGTSGQTPQLFHSQTLTTAPLPGTTP
LYPSPMTPMTPITPATPASESSGIVPQLQNIVSTVNLGCKLDLKTIALRA
RNAEYNPKRFAAVIMRIREPRTTALIFSSGKMVCTGAKSEEQSRLAARKY
ARVVQKLGFPAKFLDFKIQNMVGSCDVKFPIRLEGLVLTHQQFSSYEPEL
FPGLIYRMIKPRIVLLIFVSGKVVLTGAKVRAEIYEAFENIYPILKGFRK
TT