fruit fly ( Drosophila melanogaster )
sulf1

Western result: +

GST GST-sulf1 (fragment)

35.47 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: sulf1
CBI Antibody Core Code: A0905
"Lot" Number: 1 Position: 77(124-200)
% Identical Homology of Target to Mouse Protein: 72% (NP_758498.1| sulfatase 1; extracellular sulfatase SULF-1)
Molecular Weight of fusion protein: 35.47 KDa

Target sequence used to make this antibody:
SSPQWQATHETRSYATYLSNAGYRTGYFGKYLNKYNGSYI
PPGWREWGGLIMNSKYYNYSINLNGQKIKHGFDYAKD

Sequence Name: sulf1
Full name: sulfated
UniGene Number: N.A.
Accession Number: N.A.
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 1114
NCBI information page:

Full length sequence:
MMRHSSLRLIIGGLILLLFVLNVFSKEQGSHSHKRSHSAKRFSRDSNSAR
ERRPNIILILTDDQDVELGSLNFMPRTLRLLRDGGAEFRHAYTTTPMCCP
ARSSLLTGMYVHNHMVFTNNDNCSSPQWQATHETRSYATYLSNAGYRTGY
FGKYLNKYNGSYIPPGWREWGGLIMNSKYYNYSINLNGQKIKHGFDYAKD
YYPDLIANDSIAFLRSSKQQNQRKPVLLTMSFPAPHGPEDSAPQYSHLFF
NVTTHHTPSYDHAPNPDKQWILRVTEPMQPVHKRFTNLLMTKRLQTLQSV
DVAVERVYNELKELGELDNTYIVYTSDHGYHLGQFGLIKGKSFPFEFDVR
VPFLIRGPGIQASKVVNEIVLNVDLAPTFLDMGGVPTPQHMDGRSILPLL
LSRNRAVRDNWPDSFLIESSGRRETAEQIAESRARLQIERRNMKLANSSL
LEDFLEGAGESTTIVSSSSTAATLMSSTAQQPEDGEEEVETDNEEDDVDG
DGAMDSSAAALEEDDLDDAAFEEGDEELDQEFQQNNDLPLAPYITKMMRL
NSECSDPALLKNCLPGQKWKCVNEEGRWRKHKCKFHLQLEHQLAAMPRKQ
YQRNCACFTPDGVVYTKIRAPSAGLHRVNKRTHNGPGRRRNKREVFHTEL
PDEMEELLDLHQVVDQLVDHTHRSKRDLPASSNETIAQVIQQIQSTLEIL
ELKFNEHELHASNSSGNSYERGEKYTKSGGHRCFVDATTAKVNCSNVIYD
DEKTWRTSRTQIDMLIKLLKDKIGKLKEMKKQLRESNKQALAAGRRNDNR
RRNDQSVLDSGAGPEFNMSYFTEISSTPRSNVVGQTEVFQGYGSASAFDS
LEQTQSHRFTPRAECYCEPDVGENHADSKEMAREARRKLKEERQRKKERK
RIKKARLEKECLSEKMNCFSHDNQHWRTAPLWNDSPFCFCMNANNNTYSC
LRTINGTHNFLYCEFTTGLITFYNLTIDRFETINRAAGLTPGERSHMHDA
LDQLKSCRGRSCSIRRHQNHLEGGSSAPLLPINQVHRNNKRKHSPLAGAV
GNYAFVGPRLDMEALPPIKRRKLSKYNRLTGSQQSHMKRRPWKQTPLQQS
PRFLRTHSVTPAQA