fruit fly ( Drosophila melanogaster )
dally

Western result: +

GST GST-dally (fragment)

30.85 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: dally
CBI Antibody Core Code: A0899
"Lot" Number: 2 Position: 35(30-64)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of fusion protein: 30.85 KDa

Target sequence used to make this antibody:
DLDGIHHHQHHLHSATTHHRRRLQRDSRAKDAVGG

Sequence Name: dally
Full name: division abnormally delayed
UniGene Number: N.A.
Accession Number: N.A.
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 626
NCBI information page:

Full length sequence:
MAARSVRLAQLLLFTLLCGFVGLSAAKHLDLDGIHHHQHHLHSATTHHRR
RLQRDSRAKDAVGGSTHQCDAVKSYFESIDIKSSGTYSEKGAICGGNCCN
NATELELRDKAAGMFEQLLHHHTSSLRGVLETNAKQFQSHVLELAQISEN
MTHSLFSKVYTRMVPSSRMMIHQLYTEIMNHLIYTSNYTNSNGQLGRRGI
GSVQSNLEEAVRHFFVQLFPVAYHQMVHLSKNNLGDLHEDYVNCLQHNFD
EMHPFGDIPQQVQSNLGKSVHMSNVFMNALLQAAEVLSEADALYGEQLTD
TCKLHLLKMHYCPNCNGHHSSSRSETKLCYGYCKNVMRGCSAEYAGLLDS
PWSGVVDSLNNLVTTHILSDTGIINVIKHLQTYFSEAIMAAMHNGPELEK
KVKKTCGTPSLTPYSSGEPDARPPPHKNNVKWATDPDPGMVLFLSTIDKS
KEFYTTIVDNFCDEQQHSRDDHSCWSGDRFGDYTQLLINPGTDSQRYNPE
VPFNAKAQTGKLNELVDKLFKIRKSIGAAAPSNSIQATHDIQNDMGEGSG
GGEGQIGDDEEEYGGAHGSGDGSGDGPHTPIEESEGTTTNEVESRDSGKT
SGSNPLEGTATWMLLTLVTMLFSSCS