fruit fly ( Drosophila melanogaster )
Vps16

Western result: +

GST GST-Vps16 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Vps16
CBI Antibody Core Code: A0689
"Lot" Number: 1 Position: 100(684-783)
% Identical Homology of Target to Mouse Protein: 35% (NP_085036.2| vacuolar protein sorting 16; vacuolar protein sorting 16 (yeast homolog); clone MGC:7352)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
KEAERIRSDFKVPDRRFWWLRILTLSSQHKWEELEKLAKS
KKSPIGYDPFVEVCLKQDNVMEARKYIPRCRDNRRVVWYM
RANLFNEAIDSAFEQRDVHS

Sequence Name: Vps16
Full name: DmVps16
UniGene Number: N.A.
Accession Number: NP_649877
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 814
NCBI information page: click here

Full length sequence:
MPIMYNTGEWFKVRPDYYRKVELTTPDWPLDLDLEYMWVEVAPYGGPLAV
TRDPTKLVPVKGNARPMIRIFDTTGREMGHILWNHGKLIAMGWSDMEELI
CIQENATVFVYDMFGREKESYSIGDEASVTKIVEGKVFQSSAGTGVAVMT
TSGRVFLKQNSSKTERKLPDIPNSSMNCSCWEIVTEGRNSYCLLGRDREV
IKLFPGETVGTITANLFEKPHERIIKISVSYNHQHLALYTNTGLLWLGSV
DMRQKYCEFDTGRKDMPLQMEWIMNSDNSEADAVVISYPSYLLIVNRNAD
RSDFPYDPVMFLVAEMDGVRIITQSSHEIIQRLPKCVENIFAVNSQEPAS
YLFEAQKKFEEKSYKSDEYLSMCREKIDLAVSECIEAASYEFCPETQKSL
LRTAYFGKGFIRNHNPDEYMRIMRILRVLNTLRHERIAMPITFKQFSHLN
TEVILSRLVFRKHYAIAIQVMNDPNDTEVARKITEKFKNPSVEGISFCNI
ASKAHQAGRDDLAIKLLELESRASLHVPLLLKMRKFDRAVGSATQSGDTE
LITQVLLEMKMHMMLSNLHMTIRDHPLALNIYKKIMRESNRAGLYGIYNT
EDDQKAIAEYHFQNAIETEGLESNLSMIGNAYAQGRCTLEAELCADTSRL
IKLQKTLSTKYNVSLNGLSIHETIQELLLIGELKEAERIRSDFKVPDRRF
WWLRILTLSSQHKWEELEKLAKSKKSPIGYDPFVEVCLKQDNVMEARKYI
PRCRDNRRVVWYMRANLFNEAIDSAFEQRDVHSLFELQKNQAIVNNGTLL
SKVCDCIAMLEGK