fruit fly ( Drosophila melanogaster )
Unnamed

Western result: +

GST GST-Unnamed (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Unnamed
CBI Antibody Core Code: A0737
"Lot" Number: 1 Position: 100(549-648)
% Identical Homology of Target to Mouse Protein: 29% (NP_032667.1| microtubule associated serine/threonine kinase 2; microtubule associated testis specific serine/threonine protein kinase)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
MREKLRSDDTDSRPMGNNQDLPIDEQSRERPLSTQTSPTN
GPLPALLRAKLLAGNSNSAHCLPGSTASPAPQEQSGIFVI
DSEASPGSNGHKPKYRKGTA

Sequence Name: Unnamed
Full name: CG9753-PA
UniGene Number: N.A.
Accession Number: NM_143515
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 774
NCBI information page: click here

Full length sequence:
MSAFRYFSITDFSFEGPLLPLHAATTSKDAKDSDSPSSELNIPYTVFEVL
VAIVSIIGNVLVIIVFRRERKLRRRTNYYIVSLAMADLLVGALGIPFAIL
ASMGLPRNLHACLFTVSLLVVLCTISIFCLVAVSVDRYWAILYPMAYSRN
VRTRTAIFIISMCWVAGTIVGFLPLFGWHADVNHNQECLFVEVMDYNYLV
FLYFATIITPALLMLAFYTHIYRVIIKQVRQIVTMNPASDLSRRSSAAVV
QVTTPGRGGHTGTMLRVLGAARKRDVKATQNLSIIVLFFMICWIPLYTIN
CIKAFCPDCYVHPKLTLFCIILSHLNSAVNPVLYAYHLKDFRAALKNLLL
KMMGVDIDQQAEAIHRFSVASQHRLQSMDSNMRSTQPRLYVGEYSPIWLR
QQQEALKNSQLLPKCGVVSPCFNNINQTVAAVASVTTDLEREMWNIVEAS
SGAELGETSYEFPSPAPGSQRSSERNSSSTVPPAPPAPAKPSVPSASYDN
HNYSFSQDEDEDDDDLEFEDVFVPASSVPNPVQPGIDPVELRRSLALVMR
EKLRSDDTDSRPMGNNQDLPIDEQSRERPLSTQTSPTNGPLPALLRAKLL
AGNSNSAHCLPGSTASPAPQEQSGIFVIDSEASPGSNGHKPKYRKGTAFT
RSSLKKSRSCNCSSIAKGRGVHDEPSSNLCRDQESSVLPQHPQPANHPTE
NFFSPLRSVGSFMQHSNLFHFLQPHAARPTSSTASSTASTPTPSPPPMGQ
AQEESVPVGLTTSSPSLLATSAES