fruit fly ( Drosophila melanogaster )
TRAP80

Western result: +

GST GST-CG7957-PA (TRAP80) (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: CG7957-PA (TRAP80)
CBI Antibody Core Code: A0629
"Lot" Number: 1 Position: 100(343-442)
% Identical Homology of Target to Mouse Protein: 34% (NP_659182.1| cofactor required for Sp1 transcriptional activation, subunit 6; cofactor required for Sp1 transcriptional activation, subunit 6 (77kD))
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
CHSTTFDSSQPAPINDHDHVLEHSLHQLLREVHYKNSHHP
FPHPASAPLGPTKKRMLAGPMAADRETLLDMTKSQTILEQ
IIAQAQHIFMRKRTQYVLDT

Sequence Name: TRAP80
Full name:
UniGene Number: N.A.
Accession Number: AAF63336
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 642
NCBI information page: click here

Sequence Notes: AAF63336 is the same as NP_650686: http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=protein&list_uids=21357131&dopt=GenPept&term=&qty=1

Full length sequence:
MSNSVNISVETTCENQIREIGYDGTELYQPPPTLSESLAKCAARIDFSKT
SLDDLKKEEKSAAAAADEDKDATQFQESLWPWDAVRNKLKDALTEICVLS
DVISIAKDKRYLVLDPLLEEADDTKPIVQVYSRKKAISQAAQVLLSGAER
LRNAHSEQRNRNVSDFHIELLRLRQNWRLKKVSNAIIGDLSYRTAGSKFG
MSGTFEVTKAEETGDEDTASSSNSSSSVSGNNGMQLKASSALRVIVPAEL
QGVAYIKVITQKDQEDLCTAQLNLMGHGPNITAQVGVWQKTLEFAQNVLF
CKELFAQLAREAIQLQAPIPHVVIGNQIRATLLPNIQLIISLCHSTTFDS
SQPAPINDHDHVLEHSLHQLLREVHYKNSHHPFPHPASAPLGPTKKRMLA
GPMAADRETLLDMTKSQTILEQIIAQAQHIFMRKRTQYVLDTLARDVKDP
QIVSHWNAMNSPTMSCVKINIVTHGYDAIGRTSLVIHVKERSLKCICRDG
RVMRLSYEPQELRDLILCQINSHQISCLISLARCMSWTVLSNSNHLGIGK
VEPLGNASSCLLASPNSDRMIAVQIRCDPQIDVKVYIARSPRQDFFPSPL
VPEKLWENLGGTFKEVRFDKIEGKSFLNKMEFLMASLTSNTA