fruit fly ( Drosophila melanogaster )
skuld

Western result: +

GST GST-skuld (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: skuld
CBI Antibody Core Code: A0625
"Lot" Number: 1 Position: 100(519-618)
% Identical Homology of Target to Mouse Protein: 52% (XP_132318.6| thyroid hormone receptor associated protein 2)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
ADGDENEQNKPPQDSKQLVQQQIQQQQQRQKLWNFVDPMQ
KAPCICTKHLGNTPHGTPHGGASTYSRNSLGGDSSMPVAS
VESPATPAPSPHPNSAHSQP

Sequence Name: skuld
Full name: poils aux pattes CG9936-PB
UniGene Number: N.A.
Accession Number: NP_730589
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 2618
NCBI information page: click here

Full length sequence:
MTHQNHQTNGASLEDCHTNFYALTDLCGIKWRKFVNGERPNASSDPLADP
ILRSYSRCIQADMLCVWRRVQSTKTDHADPNALTFEMTTSTKVHPPLSLA
AAKELWIFWYGEEPDLSELVDAELLRVAANQALWNGTWKGALTYECRSLL
FKALHNLMERFVLTKDIVRFGKWFVQPCTSSDRLFGRSSQHLSFSFTFFV
HGDTVCASIDLREHPAVRPLTKEHLTEAAAAFAAASSPPGSNGSAASAGG
AVPNPGQDPNGASMDGLDGGEGAAKAAPPPHARKVMLAPFGIAGILTGNS
YKASDPIAEKILEDWASFFPLCNKDNTDVPPVVEVVSGGHKMYHPTNYVL
VTDLDDMEHMEFVEMQKMQSSVAGAAAESAVLASLSCPPGAASAPSSMGA
APSATAVPLGPASLNAPALAGAGVGATASSAAKEISRKAAPQAVSALERL
AFQPYYDQRPTSGFTFNTNNTHIPASAAVEMPERTWQDCVMNTLHVDAAA
AAAAVASSTPASGTGSLSADGDENEQNKPPQDSKQLVQQQIQQQQQRQKL
WNFVDPMQKAPCICTKHLGNTPHGTPHGGASTYSRNSLGGDSSMPVASVE
SPATPAPSPHPNSAHSQPTSVPPAEQLLNMSPHAPTSVSNLQQPPTPIDH
LLDKNTPAPTPTDQHDSKSITASPYVHQTPSVEPPSYTDHAAGGGPAGGQ
GLGTGPGSVPAQQPATPTAATSAGGAGSGGPSNAIGANAVGTISVKKLEM
QQQTPSAAMAIKQEPGAQGRGVGGVTSTTEALNNFKRLYNPPKLTLKDPD
SFYDEEWLKEVIYDFQYQEYWDYSTVKRPKMEKQRRPRYAKNLYEGQNHV
KPVMPSPGSVYGSQLLSLDESASQAGGRGGGGQAAGSSGGGLVGIANSTA
SGSDVEADGSSFFQGLNIKTEPGLHSPSCKETSKSSGGNSSGGGSGSGGN
LFTAEGLNPSLNDLEQLFETSSNDECSSVQIHTPPDSNNPSNGGCSAVTN
TIEDLKRSTAVASAAVAAAAAAAASGAGNIQAEDLTKMFPTPPSHEQQHP
NSSPCQTDVVMTDLSVDTTTTIITSSITTTCNTTITSSINTTTTACSNPS
NSIMLAAAQAPVTVVAIQTVSKMVKQEYNLELGSPMEEPINDWDYVYRPP
QQEKFVGSTRYAPLTNLPSQTQPPLTLPTGCFYQPTWSSHKSRAATLAKA
AAAQQQQHQKHQALQQRIQLHQQKLQQLQLQNQQQQQAAAAAAAAASGGV
GHQKHQHQHLHDLLSAAPRTPLTPSTVPQPLSSGGSQYLLNQLNCPQAPP
GASMQQLMHRAGMSPISPGPGMGPYAARSSPMSRATPTHPPPPYPYDLAV
ASPATSTSSYLNRPLHSQEHPHMHGLGGGATGVVGHGTGGGGHMGMVAYT
GDAGIVSGGTAMAAGSSSLLQELPEVNSVLVNILLYDTALNVFRDHNFDS
SSVCVCNADTQKIGNIRGADSGVYVPLPGVSFNPFPSGAGGALAGQRMLN
GPSSAGFGGMRMISAFGGSPASASMPGAGSGHGHGPNGGSNSSSCTPPSS
NPHITGYVDDDPVECTCGFSAVVNRRLSHRAGLFYEDEVEITGIADDPGR
NKQPTLLSIIQSLSRKNQNKQGPGETSSALDKIGAGGLPNGQLEQLGHAV
FDLLLDQCSIIQTSSSSVHRALQSHRRRMSRQRRIFGNNGAPTASLASIA
NVLEFMDAHDVISLALEQSRLAFENQRMDNMMDFHGNGSSSSHQQQQLTA
FHAPPPALRHKLAGIGAGRLTVHKWPYLPVGFTRSNKEIVRTMNAIQPML
QNAFHCKSRGGSGSKDASSYNTVSGPLTWRQFHRLAGRASGQCEPQPIPS
VVVGYEKDWISVAPHSIHYWDKFLLEPYSYARDVVYVVVCPDNEHVVNCT
RSYFRELSSTYEMCKLGKHTPIRGWDGFLQVGAARNNVPADRETTPLDDW
LRTLEHAALAEQIRRYAVAFIHQLAPYLSRVPNDKTLLNPPDGSGNSHSK
GGSSCSSNSSSVSGLPGGDLPTDNIKLEPGTEPQVQPMETNEIKQEPGVG
KGGTAAGETKPTLILGDPLGMGETLEDINPSAIVLYVVNPFTFASDSCEL
ERLALIALLRCYAELLKAVPDSVRSQMNIQIISLESVMELGPCGNRKRFS
DEIRCLALNIFSQCRRHLVHAQSVKSLTGFGTAANMEAFLKTKDEPNRRA
YKMYTAPFVLAPMHERNDKTDFSRSAGSMHGQNEHRYSVMYCNYCLSEDQ
AWLLATATDERGEMLEKICINIDVPNRARRRKAPARYVALKKLMDFIMGI
ISQTSQMWRLVIGRIGRIGHSELKSWSFLLSKQQLQKASKQFKDMCKQCT
LMYPPTILSACLVTLEPDAKLRVMPDQFTPDERFSQISMQNPLATPQDVT
CTHILVFPTSAVCAPFTRQFQNEPQVDDDFLTFEEEGNEDFSDADIGDLF
WDTHMDRVSNHGSPGRMDDNRSWQSAGGNNFKCTPPQEVEEVGSLNQQPI
SVGYMVSTAPTGRMPAWFWSACPHLEDVCPVFLKTALHLHVPSIQSADDI
LNSTNAHQSGNDHPLDSNLTADVLRFVLEGYNALSWLALDSNTHDRLSCL
PINVQTLMDLYYLTAAIA