fruit fly ( Drosophila melanogaster )
PKD-FF

Western result: +

GST GST-PKD-FF (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: PKD-FF
CBI Antibody Core Code: A0780
"Lot" Number: 2 Position: 100(284-383)
% Identical Homology of Target to Mouse Protein: 40% (NP_081033.1| RIKEN cDNA 1110020A23)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
QAGAKERDNFFREEFDDSDGDDGSEYGKYKSAMSSGVNLV
AGRPREAPKALTNAHNSPPELVNGGLVDDSSVSGGETSKS
SSDGQSEQSQSSTSSSPSAN

Sequence Name: PKD-FF
Full name: PKD-Drosophila melanogaster
UniGene Number: Dm.13712
Accession Number: AY118312
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 836
NCBI information page: click here

Full length sequence:
MEGPEVTFLFQFGSVRDAVCVPANSLTLKTLKDLACDFINTKIPDSGLTY
LSERILLFVHDYSTPNVLHIINSAAEVVDETLVEIVLTASPILYPTSEMP
TLKPHSLNVHSYKGPTFCDFCGEMLFGLVRQGLKCDGCGQNYHKRCVVKI
PNNCNRSNDATSRRSFTLQAPRSPSGSSQQSLVSTEDSTGRNDSSSSLNI
PGRHQRTHSSGSRNGLMVLRIPHTFMVHTYGIPTVCQLCKKLLKGLFKQG
LQCRDCQYNTHKKCMDKVPHDCTGEAQLSQLQIQAGAKERDNFFREEFDD
SDGDDGSEYGKYKSAMSSGVNLVAGRPREAPKALTNAHNSPPELVNGGLV
DDSSVSGGETSKSSSDGQSEQSQSSTSSSPSANIPLMRIVQSVKHTKKRG
GQALKEGWLVHYTSLDKAVKRYYWRLDSKTITLFVSEQGSKYHKELPLAE
LLSIESHLGDPRPECNYCFELRLPNLSYFVGQDPQVGAKEEQAVRLPPPD
SGIGSDIAKSWETSIRQAFMHVTNTQCCESEEQVQDMGQLYQIFPDEVLG
SGQFGVVYGGVHKKTQREVAIKVIDKLRFPTKQEAQLKNEVAILQNISHC
GVVNLERMFETPERIFVVMEKLKGDMLEMILSHARGRLSERVTKFLITQI
LIALKYLHSQNIVHCDLKPENVLLSSDAEFPQVKLCDFGYARIIGEKSFR
RSVVGTPAYLAPEVLRNKGYNRSLDMWSVGVIIYVSLSGTFPFNEEEDIN
DQIQNAAFMYPPNPWKEISSNAIDLINNLLQVKQRKRYTVDKSLLHYWLQ
DKQTYRDLRNLEAQVGAGRYLTHEADDLRWDCAGDM