fruit fly ( Drosophila melanogaster )
dK&B

Western result: ++

GST GST-dK&B (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: dK&B
CBI Antibody Core Code: A0376
"Lot" Number: 1 Position: 100(121-220)
% Identical Homology of Target to Mouse Protein: 22% (NP_035681.1| upstream binding transcription factor, RNA polymerase I; transcription factor UBF)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
WKTTDSAYLSSKCGGLRDRADRRKQQYTSPQNLEPDQTLK
YEVDSVDESRNAADFSSAFNSNDNCESAAECERSGGHNNK
EEDEDDCTHKDNKSDKDTDE

Sequence Name: dK&B
Full name: ken and barbie CG5575-PA (K&B)
UniGene Number: N.A.
Accession Number: NP_523833
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 601
NCBI information page: click here

Full length sequence:
MKEFQRMLMLQYSKHGECILKEIGAAFRGEHPADLTIVCENKVKLHAHKL
VLAAASPLIRNLLEDTHLSDCSTTVYFPDVNATYFKFLLDFLYSGQTCIT
SRDVNYLHDLLLLLQIKSDSWKTTDSAYLSSKCGGLRDRADRRKQQYTSP
QNLEPDQTLKYEVDSVDESRNAADFSSAFNSNDNCESAAECERSGGHNNK
EEDEDDCTHKDNKSDKDTDEIVNLSNAPPSGTSGSNSNISTSSNHQQQQH
HHHHHHNHNNNNNNNNNNSSSSTINPVNLSLDLRTKSENSASRTLGSGSD
HSGIDLAVTASESTKRKGLFFDSHKDVMKPLSDGSDINSSPENYVVTPHR
KRRPGFHNTQSDNQPFTSYPHSLLEELRLAKSTTSPISGFGSEKNMLAHL
EDGALNGDTLTPDRKHLLEAQRNRAQSPEIPMHLGPQFVYQWQSNQNAAM
SAMPNLQSRLSSLSHISLNLDHPEGRSGSASGSGANLAGSNTHASSVREY
RCEYCGKQFGMSWNLKTHLRVHTGEKPFACRLCVAMFKQKAHLLKHLCSV
HRNVITTTNGADTENRYSCCFCSMCFESVQELVRHLSGHHNNLLLTKNLR
E