fruit fly ( Drosophila melanogaster )
GCN5

Western result: +

GST GST-GCN5 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: GCN5
CBI Antibody Core Code: A0715
"Lot" Number: 1 Position: 100(326-425)
% Identical Homology of Target to Mouse Protein: 31% (NP_064388.1| general control of amino acid synthesis-like 2; general control of amino acid synthesis, yeast homolog-like 2; general control of amino acid synthesis-like 2 (yeast); PCAF-B/GCN5)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
SERDRFPEDKRSIITLMPKFLETLRAELLKDDSPIWDTSY
RPSNSFVIQQRKRNQEVASVPIGPSAASIGGNKRTSVGEP
LHKRIKKEPTDRPSSENLDD

Sequence Name: GCN5
Full name:
UniGene Number: N.A.
Accession Number: AAC39102
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 813
NCBI information page: click here

Full length sequence:
MSGGPSITIKSQPIDGNNTGNAAAQQQQQAANGAATAGASGAAGSAQNPG
HGGAASGAGSVPAEGTRQNSLQRIQQRKQKVFNLPVPQKLAKLSMYSACQ
SEGCRCTGWKTPQENRHRDVESSYCPEFNEECRNTSCRHSLRSHIAHLDN
ISSSSMNELLGAIIDMENLFMSMQRVEDEDTKKVYQYLFRLLRQCVLTRQ
QAVIRGPLGDPPFETPCITKAVLSLVFYKYNHLSTPELQTMTEVAKTFLN
FLNHYNFESPSTRRGDLTHEDASNYKINYTRWLVFCHVPAFCNSLRQCET
SLVFGRTLLRTVFQCVSQQLKKKCISERDRFPEDKRSIITLMPKFLETLR
AELLKDDSPIWDTSYRPSNSFVIQQRKRNQEVASVPIGPSAASIGGNKRT
SVGEPLHKRIKKEPTDRPSSENLDDLPADVVMRAMKSVSESKTTNKAEIL
FPVNVSRDENVKAEEQKRAIEFHVVGNSLTKPVDKQTVLWLLGLQLVFAY
QLPEMPREYISQLVFDTKHKTLALIKENQPIGGICFRPFPSQGFTEIVFC
AVTMSEQVKGYGTHLMNHLKDYSIQRGIKHLLTFADCDAIGYFKKQGFSK
DVKLARPVYAGYIKEYDSATLMHCELHPSIVNTQFIAVIRSQSEILKELI
AQRHNEVQKVRPGLTCFKEGLPVIPVESIPGLREIGWKPQNRPARSSRPL
EESTDPEKLATSFASVLQSVRQHTTAWPFLRPVTAAEVPDYYDHIKYPMD
LKTMGERLKKGYYQTRRLFMADMARIFSNCRFYNSPDTEYYRCANSLERY
FQTKMRELGLWDK