fruit fly ( Drosophila melanogaster )
Baf180

Western result: +

GST GST-Baf180 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Baf180
CBI Antibody Core Code: A0700
"Lot" Number: 1 Position: 100(1325-1424)
% Identical Homology of Target to Mouse Protein: 50% (NP_080123.2| polybromo 1)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
LYSSEVRKSICQSNPDATFGDISRMVGTEWKNLPSSVKQS
WEDRASRQNEETAALRRELDDVQNSASPSSQVSQDVFGQV
LTFECQWDKCDFQFEELSDC

Sequence Name: Baf180
Full name: CG11375
UniGene Number: N.A.
Accession Number: AAF56339
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 1654
NCBI information page: click here

Full length sequence:
MLSRKRRASSISSRQDEDPLQLDDSTPEQSPVQQTTTQSARKKRRLDPTE
LCQQLYDSIRNIKKEDGSMLCDTFIRVPKRRQEPSYYDVVVNPIDLLKVQ
QKLKTDSYDDLDDLMADLELLIGNAKAFYIPGSSEHQDAVSLWQHIHSQR
QRIMEANGLAEEEPRARRMSRQVRRMTSSTEPGGDGATDDEYNQYEELFA
SVMTATDPVGDRLMHRMFQLLPSKKIYPDYYDVIEHPIDLRLIATKIQMN
AYSSLAEMERDLLQMTKNACLFNEPGSQIYKDAKSLKRIFTQRRIELEMG
KGKLAKRVKSLSSAAIAALKEEVDSSDDEETSKKGEGPMWALFDHLYNAP
GTSEHPGVTGPPLGNSLWKLPVRRFHPEYFELIKRPISMSQIHTKLKKGD
YANISDLTADLYLMLDNAKKAFPTSHRTHKDALKMLKLMNAKLVEESLEE
GSDLDDEDAEEMDTEVFTVSTQPEKRKPGRPRINSNSNSNASHTPNNSNS
PKSNRIAINAAIKKKILSIQKYLVDYSLGNRRPIEMFMEKPPRKIYPDYY
DIIQNPIDMNTIEHNIRTDRYAAVEDVVSDYRLMFSNCRQYNEEGSNIYE
DANILERALNEKLKEFPGLTEGKKSQQKYSKVGRKLKTAVITERLWQFYE
TVKEYQEPKGKRQLSLIFTKLPSKSEYPDYYDIIREPIDMDRIAQKLKQG
AYDTLDDLAADFLLMLENACKYNEPDSQIYKDALVLQQLTLQLKQQLRTE
RDSLPDVPLAVQELFLTLFTTIYNHQDEEGRCYSDSLAELPEYDEIGEGP
KVRGISLDLVKRRLDKGAYKRLDVYQEDIFACLERARKLSRTDSDIFQDS
IELQTYFIRKRDELCKDTLSSSALNFSLERLMADVELCRQQKMQQEEQDQ
EQDKEKDEFNAAKGESMTINQQVFSPGDYVYVQMPENKIPSICCIERLWT
SPTNEKLMQASIFVRPHETYHVTTRKFLEKEVFKSSLSQTISMDKVQGMC
YVMNIKDYIKMRPENLPEKDVYVCESRYNIQGRWFKKLKSWPPVREGSSV
KFVPREQPLELKRVMSVFKERLEKHKGELEELKLQETLVEKEKPNVSCDP
PPNAEVGSTYYQQYNTICSGAIKTGDFVYVATQTGKQSVAQVQQIWEQNG
KSYFKGPWLLPPSETTPGLGKQFYRQELLLSTVEEVSPVVGIVGRCAVLE
YSEFISSRPTEISESDVYICESVYDELKKALRKLVTGNMRKFQHSPSVTE
DEIFYFKTPIKPSKDFKNELNELGMLEDSMDGDTPSLSSDIAAMSSPAPS
VNSTPLTSKVKAAKSAKKCLTGYILYSSEVRKSICQSNPDATFGDISRMV
GTEWKNLPSSVKQSWEDRASRQNEETAALRRELDDVQNSASPSSQVSQDV
FGQVLTFECQWDKCDFQFEELSDCTEHCMSDSTGHIQRHPQAGVETEYVC
LWRNCPRVKKSVQAFPNVLRLIKHVREVHLSKCGKTISAADRSKNFVPRR
QKHAQLATTVPIPPSLAQSPRAPSNLEAVQLQQQQHPQQNMHQLQQQQTI
VLGPPPEPLFVTVPPRTTRVIHSEAYIKYIESLQTGSHLNVVTCNNNWRR
ALTHITPAQVVAKTALPEKWIGPNLRDQGNVVQALCHLRNFMVDDILQIR
RSCN