fruit fly ( Drosophila melanogaster )
Odorant Receptor 85d

Western result: +

GST GST-Or85d (fragment)

18.84 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Or85d
CBI Antibody Core Code: A1187
"Lot" Number: 1 Position: 44 (107-150)
% Identical Homology of Target to Mouse Protein: 41% (NP_031741.1| circadian locomoter output cycles kaput)
Molecular Weight of fusion protein: 18.84 KDa

Target sequence used to make this antibody:
NISRQRKRLTQVVSRLEELHPQGLAQQEPYNIGHHLSGYS
RYSK

Sequence Name: Odorant Receptor 85d
Full name:
UniGene Number: N.A.
Accession Number: CG11742
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 412
NCBI information page:

Full length sequence:
MLTKKDTQSAKEQEKLKAIPLHSFLKYANVFYLSIGMMAYDHKYSQKWKE
VLLHWTFIAQMVNLNTVLISELIYVFLAIGKGSNFLEATMNLSFIGFVIV
GDFKIWNISRQRKRLTQVVSRLEELHPQGLAQQEPYNIGHHLSGYSRYSK
FYFGMHMVLIWTYNLYWAVYYLVCDFWLGMRQFERMLPYYCWVPWDWSTG
YSYYFMYISQNIGGQACLSGQLAADMLMCALVTLVVMHFIRLSAHIESHV
AGIGSFQHDLEFLQATVAYHQSLIHLCQDINEIFGVSLLSNFVSSSFIIC
FVGFQMTIGSKIDNLVMLVLFLFCAMVQVFMIATHAQRLVDASEQIGQAV
YNHDWFRADLRYRKMLILIIKRAQQPSRLKATMFLNISLVTVSDLLQLSY
KFFALLRTMYVN