fruit fly ( Drosophila melanogaster )
cali

Western result: +

control Cali A1153b (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: Cali
CBI Antibody Core Code: A1153b
"Lot" Number: 1 Position: 20 (625-644)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
ELTSDTDDSSRRSDTPAKIS

Western result: +

GST GST-Cali A1153a (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Cali
CBI Antibody Core Code: A1153a
"Lot" Number: 1 Position: 100 (863-962)
% Identical Homology of Target to Mouse Protein: 66% (NP_808367.1| formin homology 2 domain containing 1)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
SVLKTPRPEVVLVVTRSESLVVKALTKKRSSLGSLSSLNE
KPTELDYERKRNYHKASRSLDLDLDLVSNEAGESPVATTP
STGSVSPPQPASLHDEDAEA

Sequence Name: cali
Full name: Excalibur
UniGene Number: N.A.
Accession Number: CG9598
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 1100
NCBI information page:

Full length sequence:
MFIKLILEIVVYIHLGTLSNHHSNISSISGINASSNIPAATLISSSIKKT
STEAATTKPAAPQIPGKMELLSSNTGTTTTTTTATHHLHQATTTKQLLVQ
DYLSYASPPTYSRLPPDGHEFPPNFSEPLIMHSHPLKVTTELSYEIQKGG
KDESSAPPLPKTGPPATVPRKVYRQDLVINVEPAPSLTRDYQRSLSGGTP
RKPSDWRKDEKSEKSVRDKIAMFSSNNELDAIPPAPATAPISSSFTRKPL
NRSSENLLDSCSSSSAPSLKTRAMSVENLNDVQRQYQLAKQLPQLHVADS
MYSLNTATPTPSYASLPRRSHGGSYSSGVERRISFSGEGGDAANRKAAIT
NILEQRRRSLSKLRGLVIPERPQLLEPILDLPEIKSQVKAASGEDSTDSG
LGESHRSTVNRNCQLGAGGAANNYRSILNTNQRRPLEQQLSQPPAKPPRT
SLTPLQPRSMMIPPPPPPLDQESDTDSVFSHTARVATPPEKFALTRTLSS
ETNTSIASSNTSTLTSGSSAGSQASCSSLGSTPAVDLTRRVLKSQVINGE
AVALSSRKSILASAKCRSAKSRGQEEDNDSTDGEACSLANRRMKPISSYK
LQQQQIQLGKQLVVDKLINVAAYVELTSDTDDSSRRSDTPAKISAMFIDE
ERKASFKGDPNQQAKVKVEQVKPMVLPMLLPSAKREPLKQQTTAELREKF
ERSAAAQAQTQNHSPVIHKVTQKPHHERFSSLDSLASSSSGVSSTTQNVS
TTQETATEFGSFSSLGSNQSLITAQDVQQIVEEADPPLKTPEAFIIVLQR
ENPESSIGITLAGGSDYEAKEITIHKILSNTPAAKDGRLKKGDRILAVNG
MSMRGLTHRESISVLKTPRPEVVLVVTRSESLVVKALTKKRSSLGSLSSL
NEKPTELDYERKRNYHKASRSLDLDLDLVSNEAGESPVATTPSTGSVSPP
QPASLHDEDAEATIAGIRARRQLSRGDAAKLSTSELLERAAEARNAIAAE
IRAQAEDAAASGGGARCVEIVKDSCGLGFSIEGGFDSPLGNRPLIVKKVF
MGGAAQKTNQVRNGDEILSINGASTSRMTRVDAWNYMKQLPLGPVKICFA