fruit fly ( Drosophila melanogaster )
ari-2

Western result: +

control ari-2 (fragment)

30.3 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: ari-2
CBI Antibody Core Code: A1009a
"Lot" Number: 2 Position: 30 (11-40)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of GST-fusion: 30.3 KDa

Target sequence used to make this antibody:
SDNDGEYDDDYDYYNTGEDCDVERLDPKRA

Sequence Name: ari-2
Full name: ariadne-2
UniGene Number: N.A.
Accession Number: O76924
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 509
NCBI information page:

Full length sequence:
MDSDIEMDMESDNDGEYDDDYDYYNTGEDCDVERLDPKRADPEYFEYECL
TVEDIEKLLNERVEKLNTILQITPSLAKVLLLEHQWNNVAVVEKYRQDAN
ALLVTARIKPPSVAVTDTASTSAAAASAQLLRLGSSGYKTTASATPQYRS
QMCPVCASSQLGDKFYSLACGHSFCKDCWTIYFETQIFQGISTQIGCMAQ
MCNVRVPEDLVLTLVTRPVMRDKYQQFAFKDYVKSHPELRFCPGPNCQII
VQSSEISAKRAICKACHTGFCFRCGMDYHAPTDCQVIKKWLTKCADDSET
ANYISAHTKDCPKCHICIEKNGGCNHMQCFNCKHDFCWMCLGDWKTHGSE
YYECSRYKDNPNIANESVHVQAREALKKYLHYYERWENHSKSLKLEQQTI
DRLRQRINSKVMNGSGTWIDWQYLFNAAALLAKCRYTLQYTYPYAYYMEA
GSRKNLFEYQQAQLEAEIENLSWKIERAETTDLGDLENQMDIAEKRRTTL
LKDFFPVDA