elegant worm (C. elegans) ( Caenorhabditis elegans )
wrm-1

Western result: +

GST GST-wrm-1 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: wrm-1
CBI Antibody Core Code: A0742
"Lot" Number: 1 Position: 100(6-105)
% Identical Homology of Target to Mouse Protein: 30% (XP_486103.1| Arg/Abl-interacting protein ArgBP2)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
AETFSQPCTPLNFNPMTPSTSRVSTPVRPSSTMSARQYSG
SPFKAQPQNMEPSNSRVQELREAAVGKRSYTNAWMQGTYA
PPQMAGQQQRFSRPPSVIGS

Sequence Name: wrm-1
Full name: worm aRMadillo (90.3 kD) (wrm-1)
UniGene Number: N.A.
Accession Number: NP_498236
Source organism: elegant worm (C. elegans) ( Caenorhabditis elegans )
Full length size (aa): 796
NCBI information page: click here

Full length sequence:
MDVDCAETFSQPCTPLNFNPMTPSTSRVSTPVRPSSTMSARQYSGSPFKA
QPQNMEPSNSRVQELREAAVGKRSYTNAWMQGTYAPPQMAGQQQRFSRPP
SVIGSTMSHMTNMSEMTAYSYGGLSMLSVNTEMGEFNNFVNQAPYQRALT
RVSQVSENQDPTNRQYPMTAPEIIENLESTELINQAAAIRALEPIVKAGG
MLQTWGPKGAEPIIRALFQVLIPRPVENENVIRKAFEILHHSILLSNKEI
RRIDRMFFRLNAALMDPNGPPVFNVPKPYSIYEIVMTRAIQLDTKFESSA
MVLLVHLCCKPHFMKIFFGEDETQQSPAHRRLHKIVIEFAIGNLRRPETK
SKNKGLCVSIIKNLSNKNATIKDMSERLGVVSLFHQIMQNEVIHEDLLWS
TMQALTVFCGDVKNGTHFVQMGGAQVLCGLLSHGSTRLLHELLKCLRRVS
DLPAIQEQDMKESIHCIVQLIGCSDVTIVELATGTLRNIGLHNKMNKAFM
VQDGVTSHAIAVLRTSEQFTYQPHANIDLYRKQILSIYENCLSVLNNVTS
MAPQDIKESAVSACRMISENADSAYVLLHYFNVGNRKCRKLAVTVMKRVI
ETVPAFADPFVDLLGTTNEPLPILLLQRAFQSLDEWRKTSVEMMNCDGRS
AEQRRELDDRRKDHEDIVKRSVGLLTNLCSQANPRFFHSLKLVLTNGTLN
PFQWLTHEMSDGILQEWLAFILSICSRDESLQTFMMYRFLEQAKMTEAFF
AELKARRQNSNIQTMLSKIIDLGRHQQRIVSQQHQQHQMQHHRQLM