elegant worm (C. elegans) ( Caenorhabditis elegans )
ceHIF-1

Western result: +

GST GST-ceHIF-1 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: ceHIF-1
CBI Antibody Core Code: A0185
"Lot" Number: 1 Position: 100(400-499)
% Identical Homology of Target to Mouse Protein: 52% (NP_031949.1| ELK4, member of ETS oncogene family)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
PVVGGEEPVKQADMGARKNSYDDVLQWLFRDQPSSPPPAR
YRSADRFRTTEPSNFGSALASPDFMDSSSRTSRPKTSYGR
RAQSQGSRTTGSSSTSASAT

Sequence Name: ceHIF-1
Full name: hypoxia Inducible Factor HIF-1, mammalian HIF (hypoxia inducible factor) homolog, Helix Loop Helix containing protein
UniGene Number: N.A.
Accession Number: NP_508008
Source organism: elegant worm (C. elegans) ( Caenorhabditis elegans )
Full length size (aa): 719
NCBI information page: click here

Full length sequence:
MEDNRKRNMERRRETSRHAARDRRSKESDIFDDLKMCVPIVEEGTVTHLD
RIALLRVAATICRLRKTAGNVLENNLDNEITNEVWTEDTIAECLDGFVMI
VDSDSSILYVTESVAMYLGLTQTDLTGRALRDFLHPSDYDEFDKQSKMLH
KPRGEDTDTTGINMVLRMKTVISPRGRCLNLKSALYKSVSFLVHSKVSTG
GHVSFMQGITIPAGQGTTNANASAMTKYTESPMGAFTTRHTCDMRITFVS
DKFNYILKSELKTLMGTSFYELVHPADMMIVSKSMKELFAKGHIRTPYYR
LIAANDTLAWIQTEATTITHTTKGQKGQYVICVHYVLGIQGAEESLVVCT
DSMPAGMQVDIKKEVDDTRDYIGRQPEIVECVDFTPLIEPEDPFDTVIEP
VVGGEEPVKQADMGARKNSYDDVLQWLFRDQPSSPPPARYRSADRFRTTE
PSNFGSALASPDFMDSSSRTSRPKTSYGRRAQSQGSRTTGSSSTSASATL
PHSANYSPLAEGISQCGLNSPPSIKSGQVVYGDARSMGRSCDPSDSSRRF
SALSPSDTLNVSSTRGINPVIGSNDVFSTMPFADSIAIAERIDSSPTLTS
GEPILCDDLQWEEPDLSCLAPFVDTYDMMQMDEGLPPELQALYDLPDFTP
AVPQAPAARPVHIDRSPPAKRMHQSGPSDLDFMYTQHYQPFQQDETYWQG
QQQQNEQQPSSYSPFPMLS