elegant worm (C. elegans) ( Caenorhabditis elegans )
F14D12.4a

Western result: +

GST GST-F14D12.4a (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: F14D12.4a
CBI Antibody Core Code: A1210
"Lot" Number: 1 Position: 100 (19-118)
% Identical Homology of Target to Mouse Protein: 29% (NP_035554.1| nuclear receptor co-repressor 2; silencing mediator of retinoid acid and thyroid hormone receptor)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
GSVKVETRLVSNERSSSIQQEGAMLPSSSSKDDDLLSTSS
DEVENMATRTLQQLEESTSIISANSDDDSVKKEKQAEKDV
EKGNGKEEKANIQNEFGVCG

Sequence Name: F14D12.4a
Full name:
UniGene Number: N.A.
Accession Number: U26735
Source organism: elegant worm (C. elegans) ( Caenorhabditis elegans )
Full length size (aa): 481
NCBI information page:

Full length sequence:
MSATMSSARNSVVSLSSNGSVKVETRLVSNERSSSIQQEGAMLPSSSSKD
DDLLSTSSDEVENMATRTLQQLEESTSIISANSDDDSVKKEKQAEKDVEK
GNGKEEKANIQNEFGVCGWILTILSYLLIFFTLPISACMCIKVVQEYERA
VIFRLGRLMPGGAKGPGIFFIVPCIDTYRKVDLRVLSFEVPPQEILSKDS
VTVAVDAVVYFRISNATISVTNVEDAARSTKLLAQTTLRNILGTKTLAEM
LSDREAISHQMQTTLDEATEPWGVKVERVEVKDVRLPVQLQRAMAAEAEA
AREARAKVIVAEGEQKASRALKEAAEVIAESPSALQLRYLQTLNSISAEK
NSTIIFPFPIDLLSAFLQRTPPKVEEPPSLPKKIRSCCLYKYPDWVQGMV
GSEGGGGHGHSHGGGGGGLGSSQGAFHPSQAGSGPSTTTTSGRPLLRSMR
EAQFHSAAPPISAPNQSQTSVSQLDPALLIR