elegant worm (C. elegans) ( Caenorhabditis elegans )
wee-1.3

Western result: +

GST GST-wee-1.3 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: wee-1.3
CBI Antibody Core Code: A0998
"Lot" Number: 2 Position: 100 (251-356)
% Identical Homology of Target to Mouse Protein: 47% (NP_075545.1| membrane-associated tyrosine-and threonine-specific cdc2-inhibitory kinase; MYT1 kinase)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
INLKNPNDVKSAEEGDSKYLAPEVLNGRPTKSSDIFSLGM
TILEATTDLDVPSNGDSWHQIRNGQIPDRFFAGISTDLRS
LIALMLDSDPRIRPTSRDLL

Sequence Name: wee-1.3
Full name:
UniGene Number: N.A.
Accession Number: CAB16484
Source organism: elegant worm (C. elegans) ( Caenorhabditis elegans )
Full length size (aa): 677
NCBI information page:

Full length sequence:
mddtegnssmdsirngqssplpqvtprlpqipmmmretplstkrerqait
prfrrpapkmiktmpptrsiwsvrkesvpllvtpqgpkplespkydhtna
qsffeqvfqideiigrgsfgevfaarcredsqlyavkvslapirqhsisk
yreaeshmiipphknlvkfyraweetgrlyiqtelcdqsllkyctekhal
pedeiwnifvdllqavhhlhsndmihddikpenifltkdmicklgdfglv
inlknpndvksaeegdskylapevlngrptkssdifslgmtileattdld
vpsngdswhqirngqipdrffagistdlrslialmldsdprirptsrdll
dhpvikkklmkrgtyvkcisilngffyafsavlvwvmaffsvlfhpivrf
haaikdrqseicaqfvnnqqhtpiqtpetskvylesltgvavrqasqivs
pfdfsddenppnaqrrlftgavpcrlnfdndqdddeeqatcsssnssaie
pqldepespprmndvidklgkrgtprsarrnltfnrhrqvaasvapkssl
nhynhhtgsgdgfsnnslipisdqertekylrmrlteqqldwadqnnvid
eapppmscpprirrsirdlprmpvlnfnvldepsnkptvdhhtileqses
prrrlnrgakpvprnrmmsfgssgdev