Chinese hamster ( Cricetulus griseus )
Rad52

Western result: +/-

GST GST-Rad52 (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Rad52
CBI Antibody Core Code: A1227
"Lot" Number: 1 Position: 100 (187-286)
% Identical Homology of Target to Mouse Protein: 82% (NP_035366.1| RAD52 homolog; RAD52 homolog, (S. cerevisiae))
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
VDLTKAKREDFEPSVEQARYNSCLQNEAPGPPKPQEAASP
CRPSHPHDSNIRLQGAKDCSSSCSLAAPMESDAIHQRKLR
KLRQKQLQQQFRQQMEAHLQ

Sequence Name: Rad52
Full name:
UniGene Number: N.A.
Accession Number: AF220495.1
Source organism: Chinese hamster ( Cricetulus griseus )
Full length size (aa): 418
NCBI information page: click here

Full length sequence:
MAGPEEAVRGGCDTHPPFAGGKSVLCFGQSQYTAEEYQAIQRALRQRLGP
EYISSRMAGGGQKVCYIEGHRVINLANEMFGYNGWAHSITQQNVDFVDFN
NGKFYVGVCAFVRVQLKDGSYHEDVGYGVSEGLRSKALSLEKARKEAVTD
GLKRALRSFGNALGNCILDKDYLRSLNKLPRQLPLEVDLTKAKREDFEPS
VEQARYNSCLQNEAPGPPKPQEAASPCRPSHPHDSNIRLQGAKDCSSSCS
LAAPMESDAIHQRKLRKLRQKQLQQQFRQQMEAHLQGHTPAVKVKAEREA
VLPDLPPKHSTPVTAASELLREKAIFPDNPEDNLEMWDLTPDLEDIIKPL
SRPEPPQTSATRVQVIQDGVLHGLCHQMPPEKHEAGHLQAHSTHQHVLGN
SDSHRKSQDLKKRKGDPS