Sulfolobus solfataricus
TFB-1

Western result: +

GST GST-TFB-1 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: TFB-1
CBI Antibody Core Code: A0823
"Lot" Number: 2 Position: 100(128-227)
% Identical Homology of Target to Mouse Protein: 29% (NP_663521.1| general transcription factor IIB)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
ERIGNLLNLPKSVKDEAALIYRKAVEKGLVRGRSIESVVA
AAIYAACRRMKLARTLDEIAQYTKANRKEVARCYRLLLRE
LDVSVPVSDPKDYVTRIANL

Sequence Name: TFB-1
Full name: SSO0446; Transcription initiation factor IIB (TFIIB) homolog (TFB-1)
UniGene Number: N.A.
Accession Number: 15897377; NP_341982
Source organism: Sulfolobus solfataricus
Full length size (aa): 309
NCBI information page:

Full length sequence:
MLYLSEENKSVSTPCPPDKIIFDAERGEYICSETGEVLEDKIIDQGPEWR
AFTPEEKEKRSRVGGPLNNTIHDRGLSTLIDWKDKDAMGRTLDPKRRLEA
LRWRKWQIRARIQSSIDRNLAQAMNELERIGNLLNLPKSVKDEAALIYRK
AVEKGLVRGRSIESVVAAAIYAACRRMKLARTLDEIAQYTKANRKEVARC
YRLLLRELDVSVPVSDPKDYVTRIANLLGLSGAVMKTAAEIIDKAKGSGL
TAGKDPAGLAAAAIYIASLLHDERRTQKEIAQVAGVTEVTVRNRYKELTQ
ELKISIPTQ