Streptococcus pyogenes
hyaluronidase

Western result: +

GST GST-hylA (fragment)

23.68 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: hylA
CBI Antibody Core Code: A1246
"Lot" Number: 1 Position: 88 (33-120)
% Identical Homology of Target to Mouse Protein: 28% (NP_079982.2| TBC1 domain family, member 15)
Molecular Weight of fusion protein: 23.68 KDa

Target sequence used to make this antibody:
ADTLTSNSEPNNTYFQTQTLTTTDSEKKVVQPQQKDYYTE
LLDQWNSIIAGNDAYDKTNPDMVTFHNKAEKDAQNIIKSY
QGLDHENR

Sequence Name: hyaluronidase
Full name:
UniGene Number: N.A.
Accession Number: N.A.
Source organism: Streptococcus pyogenes
Full length size (aa): 868
NCBI information page:

Full length sequence:
mntyfcthhkqlllysnlflsfammgqgtaiyadtltsnsepnntyfqtq
tltttdsekkvvqpqqkdyytelldqwnsiiagndaydktnpdmvtfhnk
aekdaqniiksyqgldhenrtylwehakdysasanitktyrniekiakqi
tnpescyyqdskaiaivkdgmafmyehaynldrenhqttgkenkenwwdy
eigtprainntlslmypyftqeeilkytapiekfvpdptrfrvraanfpp
feansgnlidmgrvklisgilrkddleisdtikaiekvftlvdegngfyq
dgslidhvvtnaqsplykkgiaytgaygnvlidglsqlipiiqktkspie
adkmatiyhwinhsffpiivrgemmdmtrgrsisrfnaqshvagiealra
ilriadmseephrlalktriktlvtqgnvfynvydnlktyhdiklmkell
sdtsvpvqkldsyvasfnsmdklalynnkhdfafglsmfsnrtqnyeamn
nenlhgwftsdgmfylynndlghysenywatvnpyrlpgtteteqkpleg
tpenikpnyqqvgmtslsddafvaskklnnksalaamtftnwnksltlnk
gwfilgnkiifvgsniknqsshkayttieqrkenqkhpycsyvnnqpvdl
nnqlvdftntksiflesddpaqnigyyffkpttlsiskalqtgkwqnika
ddkspeaikevsdtfitimqnhtqdgdryaymmlpnmtrqefetyiskld
idllenndklaavydhdsqqmhvihyekkattfsnhnlshqgfysfphpv
kqnqqqkfahqgiaaknnalnshkiphkrqrrlprtgyqsssleflggal
vasfnhitkpsrkkdlri