| Western result: + | | | | | | | | The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST. | | Antibody Name/Abbreviation: Btg2 CBI Antibody Core Code: A0881 "Lot" Number: 1 Position: 100(21-120) % Identical Homology of Target to Mouse Protein: 100% (NP_031596.1| B-cell translocation gene 2, anti-proliferative) Molecular Weight of fusion protein: 38 KDa Target sequence used to make this antibody: SSLLRTRGCVSEQRLKVFSRALQDALTDHYKHHWFPEKPS
KGSGYRCIRINHKMDPIISKVASQIGLSQPQLHRLLPSEL
TLWVDPYEVSYRIGEDGSIC
| | Sequence Name: Btg2 Full name: B-cell translocation gene 2 UniGene Number: N.A. Accession Number: NM_007570 Source organism: mouse ( Mus musculus ) Full length size (aa): 158 NCBI information page: Full length sequence: MSHGKRTDMLPEIAAAVGFLSSLLRTRGCVSEQRLKVFSRALQDALTDHY
KHHWFPEKPSKGSGYRCIRINHKMDPIISKVASQIGLSQPQLHRLLPSEL
TLWVDPYEVSYRIGEDGSICVLYEEAPVAASYGLLTCKNQMMLGRSSPSK
NYVMAVSS
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