mouse ( Mus musculus )
RikG431004K08

Western result: +

GST GST-RikG431004K08 (other region) (fragment)

27.44 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: RikG431004K08 (other region)
CBI Antibody Core Code: A0765
"Lot" Number: 1 Position: 90(fuse 1021-1033|1263-1274|1423-1436|1583-1598|1622-1635)
% Identical Homology of Target to Mouse Protein: 100%
Molecular Weight of fusion protein: 27.44 KDa

Target sequence used to make this antibody:
To be posted

Sequence Name: RikG431004K08
Full name: RIKEN full-length enriched library, clone:G431004K08 product:HSGCN1 (FRAGMENT)
UniGene Number: N.A.
Accession Number: NP_766307
Source organism: mouse ( Mus musculus )
Full length size (aa): 2671
NCBI information page: click here

Full length sequence:
maadtqvsetlkrfavkvttasvkerreilselgrciagkdlpegavkgl
cklfcltlhryrdaasrralqaaiqqlaeaqpeataknllhslqssgvgs
kacvpskssgsaallaltwtcllvrivfplkakrqgdiwnklvevqclll
levlggshkhavdgavkkltklwkenpglveqyfsailslepsqnyaaml
gllvqfctnhkemdavsqhkstllefyvknilmskakppkylldncapll
rfmshsefkdlilptiqksllrspenvietissllasvtldlsqyaldiv
kglanqlksnsprlmdeavlalrnlarqcsdssatealtkhlfailggse
gkltiiaqkmsvlsgigslshhvvsgpsgqvlngcvaelfipflqqevhe
gtlvhavsilalwcnrfttevpkkltdwfkkvfslktstsavrhaylqcm
lasfrgdtllqaldflpllmqtvekaasqgtqvptvtegvaaalllskls
vadaqaeaklsgfwqlvvdekrqtftsekflllasedalctvlrlterlf
ldhphrltnskvqqyyrvlvavllsrtwhvrrqaqqtvrkllsslggvkl
anglldelktvlnshkvlplealvtdagevtemgktyvpprvlqealcvi
sgvpglkgdipsteqlaqemliishhpslvavqsglwpalltrmkidpda
fitrhldqiiprittqsplnqssmnamgslsvlspdrvlpqlistitasv
qnpalclvtreefsimqtpagelfdksiiqsaqqdsikkanmkrenkays
fkeqiiemelkeeikkkkgikeevqltskqkemlqaqmdkeaqirrrlqe
ldgeleaalglldaimarnpcgliqyipvlvdaflpllksplaaprvkgp
flslaacvmpprlktlgtlvshvtlrllkpecaldkswcqeelpvavrra
vsllhthtipsrvgkgepdaaplsapafslvfpmlkmvltempyhseeee
eqmaqilqiltvhaqlraspdtppervdengpellprvamlrlltwvigt
gsprlqvlasdtltalcasssgedgcafaeqeevdvllaalqspcasvre
talrglmelrlvlpspdtdeksglsllrrlwvikfdkedeirklaerlws
tmgldlqsdlcslliddviyheaavrqagaealsqavaryqrqaaevmgr
lmeiyqeklyrpppvldalgrvisesppdqwearcglalalnklsqylds
sqvkplfqffvpdalndrnpdvrkcmldaalatlnahgkenvnsllpvfe
eflkdapndasydavrqsvvvlmgslakhldksdpkvkpivakliaalst
psqqvqesvasclpplvpavkedaggmiqrlmqqllesdkyaerkgaayg
laglvkglgilslkqqemmaaltdaiqdkknfrrregalfafemlctmlg
klfepyvvhvlphlllcfgdgnqyvreaaddcakavmsnlsahgvklvlp
sllaaleeeswrtkagsvellgamaycapkqlssclpnivpkltevltds
hvkvqkagqqalrqigsvirnpeilaiapvlldaltdpsrktqkclqtll
dtkfvhfidapslalimpivqrafqdrstdtrkmaaqiignmysltdqkd
lapylpsvtpglkaslldpvpevrtvsakalgamvkgmgescfedllpwl
metltyeqssvdrsgaaqglaevmaglgvekleklmpeivataskvdiap
hvrdgyimmfnylpitfgdkftpyvgpiipcilkaladenefvrdtalra
gqrvismyaetaialllpqleqglfddlwrirfssvqllgdllfhisgvt
gkmttetaseddnfgtaqsnkaiitalgvdrrnrvlaglymgrsdtqlvv
rqaslhvwkivvsntprtlreilptlfglllgflastcadkrtiaartlg
dlvrklgekilpeiipileeglrsqksderqgvciglseimkstsrdavl
ffseslvptarkalcdpleevreaaaktfeqlhstighqaledilpfllk
qlddeevsefaldglkqvmavksrvvlpylvpklttppvntrvlaflssv
agdaltrhlgvilpavmlalkeklgtpdeqlemancqavilsveddtghr
iiiedlleatrspevgmrqaaaiilnmycsrskadysshlrslvsglirl
fndsspvvleeswdalnaitkkldagnqlalieelhkeirfigneckgeh
vpgfclpkrgvtsilpvlregvltgspeqkeeaakglglvirltsadalr
psvvsitgplirilgdrfnwtvkaalletlslllgkvgialkpflpqlqt
tftkalqdsnrgvrlkaadalgklisihvkvdplftellngiravedpgi
rdtmlqalrfviqgagskvdaairknlvslllsmlghdedntristagcl
gelcafltdeelntvlqqclladvsgidwmvrhgrslalsvavnvapsrl
cagrysnevqdmilsnavadripiamsgirgmgflmkyhietgsgqlppr
lssllikclqnpcsdirlvaekmiwwankeprpplepqtikpilkalldn
tkdkntvvraysdqaivnllkmrrgeellqslskildvaslealnecsrr
slrklacqadsveqvddtilt