mouse ( Mus musculus )
mWNK4

Antibody Name/Abbreviation: mWNK4
CBI Antibody Core Code: A0345
"Lot" Number: 3 Position: 100(591-690)
% Identical Homology of Target to Mouse Protein: 69% (NP_783569.1| protein kinase, lysine deficient 4)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
FLDASDPALQPPGGLPSSPAESHLCLPSGFALSIPRSGPG
SDFSPGDSYASDAASGLSDMGEGGQMRKNPVKTLRRRPRS
RLRVTSVSDQSDRVVECQLQ

Other western:
Code: A0345
Lot: 2

Western result: +

GST GST-mWNK4 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: mWNK4
Full name: PRKWNK4
UniGene Number: N.A.
Accession Number: AY187027
Source organism: mouse ( Mus musculus )
Full length size (aa): 1222
NCBI information page: click here

Full length sequence:
MLAPRNTETGVPMSQTEADLALRPSPALTSTGPTRLGPPPRRVRRFSGKA
EPRPRSSRPSRRSSVDLGLLSSWSQPASLLPEPPDPPDSAGPTRSPPSSS
KEPPEGTWMGAAPVKAVDSACPELTGSSGGPGSREPPRVPDAAARERRRE
QEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWC
ELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLV
TELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDL
KCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYD
EAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMP
EVKEIIEGCIRTDKNERFTIQDLLAHAFFREERGVHVELAEEDDGEKPGL
KLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADY
QPVARAVRERVAAIQRKREKLRKARELEVLPPDSGPPPATVSLAPGPPSA
FPPEPEEPEADQHQSFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQ
PPGGLPSSPAESHLCLPSGFALSIPRSGPGSDFSPGDSYASDAASGLSDM
GEGGQMRKNPVKTLRRRPRSRLRVTSVSDQSDRVVECQLQTHNSKMVTFR
FDLDGDSPEEIAAAMVYNEFILPSERDGFLSRIREIIQRVETLLKRDAGP
PEAAEDALSPQEEPAALPALPGPPNAEPQRSISPEQRSWAAFSTSPSSPG
TPLSPGAPFSPGTPPVFPCPIFPITSPSCYPCPFSQVSSNPYPQAPSSLL
PLSSSASQVPLPSSSLPISAPLPFSPSYPQDPLSPTSLPVCPSPPSLPST
TAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPLSLA
SCDQESLSAQTAETENEASRNPAQPLLGDARLAPISEEGKPQLVGRFQVT
SSKEPAEPPLQPASPTLSRSLKLPSPPLTSESSDTEDSAAGGPETREALA
ESDRAAEGLGVAVDDEKDEGKEPLLGGSSPILSHPSPVWMNYSYSSLCLS
SEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKKEIEDLYSRLGKQP
PPGIVAPAAMLSCRQRRLSKGSFPTSRRNSLQRSDLPGPGIMRRNSLSGS
STGSQEQRASKGVTFAGDIGRM