mouse ( Mus musculus )
Ubiquitin

Western result: +++

GST GST-Ubiquitin A0026m (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Ubiquitin
CBI Antibody Core Code: A0026m
"Lot" Number: 1 Position: full length
% Identical Homology of Target to Mouse Protein: 100% (NP_063936.1| ubiquitin A-52 residue ribosomal protein fusion product 1; ubiquitin/60S ribosomal fusion protein)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQ
QRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRG

Antibody Name/Abbreviation: Ubiquitin (C terminus)
CBI Antibody Core Code: 1Ab108
"Lot" Number: 1 Position: To be posted
% Identical Homology of Target to Mouse Protein: 100%
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
To be posted

Sequence Name: Ubiquitin
Full name:
UniGene Number: N.A.
Accession Number: N.A.
Source organism: mouse ( Mus musculus )
Full length size (aa): To be posted
NCBI information page: click here

Sequence Notes: 100% the same for both human and mouse.

This antibody reacts very poorly with ubiquitin on a western blot. It DOES perform well on ELISAs and immunoprecipitations.

NOTE: To make the Western blot shown here, instead of 50ng of ubiquitin we loaded 5ug. Ant

Full length sequence:
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE
NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR
GGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGK
QLEDGRTLSDYNIQKESTLHLVLRLRGGC