mouse ( Mus musculus )
SIM1

Western result: +

GST GST-SIM1 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: SIM1
CBI Antibody Core Code: A0753
"Lot" Number: 3 Position: 100(349-448)
% Identical Homology of Target to Mouse Protein: 78% (NP_035506.1| single-minded homolog 1)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
SKPTFSYTSSSTPTISDNRKGAKSRLSSSKSKSRTSPYPQ
YSGFHTERSESDHDSQWGGSPLTDTASPQLLDPERPGSQH
ELSCAYRQFPDRSSLCYGFA

Other western:
Code: A0753
Lot: 2

Western result: +

GST GST-SIM1 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: SIM1
Full name: single-minded homolog 1
UniGene Number: N.A.
Accession Number: NP_035506
Source organism: mouse ( Mus musculus )
Full length size (aa): 695
NCBI information page: click here

Full length sequence:
MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYL
KMRVVFPEGLGEAWGHTSRTSPLDNVGRELGSHLLQTLDGFIFVVAPDGK
IMYISETASVHLGLSQVELTGNSIYEYIHPADLDEMTAVLTAHQPYHSHF
VQEYEIERSFFLRMKCVLAKRNAGLCGGYKVIHCSGYLKIRQYSLDMSPF
DGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAE
LTGYEPQDLIEKTLYHHVHGCDTFHLRCAHHLLLVKGQVTTKYYRFLAKQ
GGWVWVQSYATIVHNSRSSRRHCIVSVNYVLTDTEYKGLQLSLDQISASK
PTFSYTSSSTPTISDNRKGAKSRLSSSKSKSRTSPYPQYSGFHTERSESD
HDSQWGGSPLTDTASPQLLDPERPGSQHELSCAYRQFPDRSSLCYGFALD
HSRLVEDRHFHTQACEGGRCEAGRYFLGPPPTGRDPWWGSRAALPLTKAS
PESREAYENSMPHITSIHRIHGRGHWDEDSVVSSPSPGSASESGDRYRTE
QYQNSPHEPSKIETLIRATQQMIKEEENRLQLRKAPPDQLASINGAGKKH
SLCFANYQQPPPTGEVCHSSALASTSPCDHIQQREGKMLSPHENDYDNSP
TALSRISSPSSDRITKSSLILAKDYLHSDMSPHQTAGDHPAISPN