mouse ( Mus musculus )
ObR

Western result: +

GST GST-ObR (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: ObR
CBI Antibody Core Code: A0599
"Lot" Number: 3 Position: 100(85-184)
% Identical Homology of Target to Mouse Protein: 100% (NP_034834.1| leptin receptor isoform 2; OB receptor; leptin receptor gene-related protein)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
TVFHCCFGNEQGQNCSALTDNTEGKTLASVVKASVFRQLG
VNWDIECWMKGDLTLFICHMEPLPKNPFKNYDSKVHLLYD
LPEVIDDSPLPPLKDSFQTV

Other western:
Code: A0599
Lot: 2

Western result: +

GST GST-ObR (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: ObR
Full name: leptin receptor isoform 1; obese-like; diabetes; leptin receptor gene-related protein; OB receptor
UniGene Number: N.A.
Accession Number: NP_666258
Source organism: mouse ( Mus musculus )
Full length size (aa): 1174
NCBI information page: click here

Full length sequence:
MMCQKFYVVLLHWEFLYVIAALNLAYPISPWKFKLFCGPPNTTDDSFLSP
AGAPNNASALKGASEAIVEAKFNSSGIYVPELSKTVFHCCFGNEQGQNCS
ALTDNTEGKTLASVVKASVFRQLGVNWDIECWMKGDLTLFICHMEPLPKN
PFKNYDSKVHLLYDLPEVIDDSPLPPLKDSFQTVQCNCSLRGCECHVPVP
RAKLNYALLMYLEITSAGVSFQSPLMSLQPMLVVKPDPPLGLHMEVTDDG
NLKISWDSQTMAPFPLQYQVKYLENSTIVREAAEIVSATSLLVDSVLPGS
SYEVQVRSKRLDGSGVWSDWSSPQVFTTQDVVYFPPKILTSVGSNASFHC
IYKNENQIISSKQIVWWRNLAEKIPEIQYSIVSDRVSKVTFSNLKATRPR
GKFTYDAVYCCNEQACHHRYAELYVIDVNINISCETDGYLTKMTCRWSPS
TIQSLVGSTVQLRYHRRSLYCPDSPSIHPTSEPKNCVLQRDGFYECVFQP
IFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSNVKAEITVNTG
LLKVSWEKPVFPENNLQFQIRYGLSGKEIQWKTHEVFDAKSKSASLLVSD
LCAVYVVQVRCRRLDGLGYWSNWSSPAYTLVMDVKVPMRGPEFWRKMDGD
VTKKERNVTLLWKPLTKNDSLCSVRRYVVKHRTAHNGTWSEDVGNRTNLT
FLWTEPAHTVTVLAVNSLGASLVNFNLTFSWPMSKVSAVESLSAYPLSSS
CVILSWTLSPDDYSLLYLVIEWKILNEDDGMKWLRIPSNVKKFYIHDNFI
PIEKYQFSLYPVFMEGVGKPKIINGFTKDAIDKQQNDAGLYVIVPIIISS
CVLLLGTLLISHQRMKKLFWDDVPNPKNCSWAQGLNFQKPETFEHLFTKH
AESVIFGPLLLEPEPISEEISVDTAWKNKDEMVPAAMVSLLLTTPDPESS
SICISDQCNSANFSGSQSTQVTCEDECQRQPSVKYATLVSNDKLVETDEE
QGFIHSPVSNCISSNHSPLRQSFSSSSWETEAQTFFLLSDQQPTMISPQL
SFSGLDELLELEGSFPEENHREKSVCYLGVTSVNRRESGVLLTGEAGILC
TFPAQCLFSDIRILQERCSHFVENNLSLGTSGENFVPYMPQFQTCSTHSH
KIMENKMCDLTVQSHPKSLKVPFQ