mouse ( Mus musculus )
Ns-pending

Western result: +

GST GST-Ns-pending (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Ns-pending
CBI Antibody Core Code: A0696
"Lot" Number: 1 Position: 100(40-139)
% Identical Homology of Target to Mouse Protein: 51% (NP_849174.1| EST C77032 short isoform; nucleostemin)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
GHKKPRKDPGVPNSAPFKEALLREAELRKQQLEELKQQQK
LDRQKEQERKRKLEVSPGDEQSNVETREESDEPKRKKAKA
GKQNPKKLHCQELKKVIEAS

Sequence Name: Ns-pending
Full name: nucleostemin, nucleostemin (Ns-pending), transcript variant 1
UniGene Number: N.A.
Accession Number: NM_153547
Source organism: mouse ( Mus musculus )
Full length size (aa): 538
NCBI information page: click here

Full length sequence:
MKRPKLKKASKRMTCHKRYKIQKKVREHHRKLRKEAKKRGHKKPRKDPGV
PNSAPFKEALLREAELRKQQLEELKQQQKLDRQKEQERKRKLEVSPGDEQ
SNVETREESDEPKRKKAKAGKQNPKKLHCQELKKVIEASDIVLEVLDARD
PLGCRCPQIEEAVIQSGSKKLILVLNKSDLVPKENLENWLNYLNKELPTV
VFKASTNLKNRKTFKIKKKKVVPFQSKICCGKEALWKLLGDFQQSCGKDI
QVGVIGFPNVGKSSVINSLKQEWICNVGISMGLTRSMQIVPLDKQITIID
SPCLIISPCNSPTALALRSPASIEELRPLEAASAILSQADNEQVVLKYTV
PEYKDSLHFFTKLAQRRGLHQKGGSPNVESAAKLVWSEWTGASLGYYCHP
PASWNHSLHFNENIAAVMKKGFNLEELEKNNAHSIQVLKGPHLTNRILFR
SSGLTNGILDEKDIVEESPRQTEDKQDADDQENGSGERNAEISDVAPVEE
TRELSPEQSTAGKPSDGSSALDRASQEDETYDFTTDYI