mouse ( Mus musculus )
mKRP-1

Western result: +

GST GST-mKRP-1 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: mKRP-1
CBI Antibody Core Code: A0373
"Lot" Number: 2 Position: 100(267-366)
% Identical Homology of Target to Mouse Protein: 100% (XP_147132.3| kelch repeat and BTB (POZ) domain containing 5)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
TLRKKKKEKGEQTARAKEANQGTEDTKAEDDEERVLPGIL
NDTLRFGMFLQDLIFMISEEGAVAYDPAANECYCASLSTQ
IPKNHVSLVTKENQVFVAGG

Other western:
Code: A0373
Lot: 1

Western result: +++

GST GST-mKRP-1 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: mKRP-1
Full name: mKRP-1 (2310024D23 RIK); adult male tongue?
UniGene Number: N.A.
Accession Number: AK009491
Source organism: mouse ( Mus musculus )
Full length size (aa): 621
NCBI information page: click here

Full length sequence:
MTLGLEQAEEQRLYQQTLLQDGLKDMLDHGKFLDCVVRVGEREFPCHRLV
LAACSPYFRARFLAEPDSAGEVRLEEVSPDVVSQVLHYLYTSEIALDEAS
VQDLFAAAHRFQIPSIFTICVSFLQKRLCLANCLAVFRLGLLLDCARLAV
AARDFICARFPLVARDNDFLGLSADELIAIISSDGLNVEKEEAVFEAVMR
WASSGDAEAQAERQRALPTVFESVRCRLLPRAFLETRVERHPLVRSQPEL
LRKVQMVKDAHEGRLTTLRKKKKEKGEQTARAKEANQGTEDTKAEDDEER
VLPGILNDTLRFGMFLQDLIFMISEEGAVAYDPAANECYCASLSTQIPKN
HVSLVTKENQVFVAGGLFYNEDNKEDPMSAYFLQFDHLDSEWLGMPPLPS
PRCLFGLGEALNAIYVVGGRELKDSEDSLDSVLCYDRLSFKWGESDPLPY
AVYGHTVLSHMDLVYVIGGKGKDRKCLNKMCVYDPKKFEWKELAPMQTAR
SLFGATVHDGRIFVAAGVTDTGLTSSSEVYSIADNKWTSFEAFPQERSSL
SLVSLAGTLYALGGFATLETESGELVPTELNDIWRYNEDEKKWEGVLREI
AYAAGATFLPVRLNVVRLTKM