mouse ( Mus musculus )
INSM1

Western result: +

control INSM1 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: INSM1
CBI Antibody Core Code: A0752
"Lot" Number: 1 Position: 20(328-347)
% Identical Homology of Target to Mouse Protein: 100% (NP_058585.1| insulinoma-associated 1; insulinoma-associated protein (IA-1))
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
PRGGPRARTRSRHQGRSARS

Sequence Name: INSM1
Full name: insulinoma-associated 1; insulinoma-associated protein (IA-1)
UniGene Number: N.A.
Accession Number: NP_058585
Source organism: mouse ( Mus musculus )
Full length size (aa): 521
NCBI information page: click here

Full length sequence:
MPRGFLVKRSKKSTPVSYRVRGGEDSDRALLLSPGCGGARAEPPVPSPGP
LPPPPPPARAERAHAALAAALACAPGPPPPPPPGPRAAHFGNPEAAHPAP
LYSPTRPVSREHEKHKYFERSFNLGSPVSAESFPTPAALLAGGGSGANGA
GGGGGGTCGGDALLFAPAELKMGTAFSAGAEAARGPGTGPPLSPAAALRP
PGKRPAPPAAVATEPPAKAAKAPSAKKPKAIRKLHFEDEVTTSPVLGLKI
KEGPLEAPRGRAGGAARPLGEFICQLCKEEYADPFALAQHKCSRIVRVEY
RCPECAKVFSCPANLASHRRWHKPRPVPRGGPRARTRSRHQGRSARSCGR
RQHQSGHAEPWRRIRIRLRDRLYECHHCAKKFRRQAYLRKHLLAHHQALQ
AKGAPPPPPPPPPPAEDILAFYAGPDEKAPHEASGDSEAAGVLGLNATAQ
CHLFPLCGETFPTKGAQERHLRLLHAAQVFPCKYCPATFYSSPGLTRHIN
KCHPSENRQVILLQVPVRPAC