mouse ( Mus musculus )
mIgG2bCh-M

Western result: +

GST GST-mIgG2bCh-M (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: mIgG2bCh-M
CBI Antibody Core Code: A0252
"Lot" Number: 3 Position: 100(101-200)
% Identical Homology of Target to Mouse Protein: 86% (NP_997616.1| expressed sequence AU044919; gamma-2b-immunoglobulin)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
GPISTINPCPPCKECHKCAPNLEGGPSVFIFPPNIKDVLM
ISLTPKVTCVVVDVSEDDPDVQISWFVNNVEVHTAQTQTH
REDYNSTIRVVSTLPIQHQD

Sequence Name: mIgG2bCh-M
Full name: IgG2b heavy chain, constent region
UniGene Number: N.A.
Accession Number: A02359
Source organism: mouse ( Mus musculus )
Full length size (aa): 335
NCBI information page: click here

Full length sequence:
AKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSV
HTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEPS
GPISTINPCPPCKECHKCAPNLEGGPSVFIFPPNIKDVLMISLTPKVTCV
VVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTIRVVSTLPIQHQD
WMSGKEFKCKVNNKDLPSPIERTISKIKGLVRAPQVYILPPPAEQLSRKD
VSLTCLVVGFNPGDISVEWTSNGHTEENYKDTAPVLDSDGSYFIYSKLNM
KTSKWEKTDSFSCNVRHEGLKNYYLKKTISRSPGK