mouse ( Mus musculus )
mHRP-2 (or mHdgfrp2)

Western result: +

GST GST-mHRP-2 (or mHdgfrp2) (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: mHRP-2 (or mHdgfrp2)
CBI Antibody Core Code: A0399
"Lot" Number: 2 Position: 100(570-669)
% Identical Homology of Target to Mouse Protein: 100% (NP_032259.1| hepatoma-derived growth factor-related protein 2)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
EKLEEQPGEQAPRELAEDEPSTDRSAPVNGEATSQKGENM
EDRAQEDGQDSEDGPRGGSSEELHDSPRDNSDPAKPGNER
QDHERTRLASESANDDNEDS

Other western:
Code: A0399
Lot: 1

Western result: ++

GST GST-mHRP-2 (or mHdgfrp2) (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: mHRP-2 (or mHdgfrp2)
Full name: heptoma-derived growth factor, related protein2
UniGene Number: N.A.
Accession Number: NM_008233
Source organism: mouse ( Mus musculus )
Full length size (aa): 669
NCBI information page: click here

Full length sequence:
MPHAFKPGDLVFAKMKGYPHWPARIDDIADGAVKPPPNKYPIFFFGTHET
AFLGPKDLFPYDKCKDKYGKPNKRKGFNEGLWEIQNNPHASYSAPPPVSS
SDSEAPEADLGCGSDVDKDKESRRVMTVTAVTTTATSDRMESDSDSDKSS
DHSGLKRKTPVLKVSVSKRARRASSDLDQASVSPSEEDSESPSESEKTSD
QDFTPEKKTAARPPRRGPLGGRKKKKVPSASDSDSKADSDGAKEEPVVTA
QPSPSSSSSSSSSSSSDSDVSVKKPPRGRKPAEKPPPKPRGRRPKPERPP
STSSSDSDSDSGEVDRISEWKRRDEERRRELEARRRREQEEELRRLREQE
REEKERRKERAERGGSSGEELEDEEPVKKRSRKARGRGTPSSSDSEPEGE
LGKEGKKLAKKSQLPGSESARKPGQKEKRGRPDEKPRARPVKVERTRKRS
EGLSLERKGEKKKEPSVEERLQKLHSEIKFALKVDNPDVRKCLSALEELG
TLQVTSQILQKNTDVVATLKKIRRYKANKDVMAKAAEVYTRLKSRVLGPK
VEALQKVNKAGAEKERADNEKLEEQPGEQAPRELAEDEPSTDRSAPVNGE
ATSQKGENMEDRAQEDGQDSEDGPRGGSSEELHDSPRDNSDPAKPGNERQ
DHERTRLASESANDDNEDS