mouse ( Mus musculus )
FGA

Western result: +

GST GST-FGA (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: FGA
CBI Antibody Core Code: A0412
"Lot" Number: 2 Position: 100(268-367)
% Identical Homology of Target to Mouse Protein: 62% (NP_034326.1| fibrinogen, alpha polypeptide; fibrinogen A alpha polypeptide; fibrinogen, A alpha polypeptide)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
DGGSRGDSPGDSRGDSRGDFATRGPGSKAENPTNPGPGGS
GYWRPGNSGSGSDGNRNPGTTGSDGTGDWGTGSPRPGSDS
GNFRPANPNWGVFSEFGDSS

Other western:
Code: A0412
Lot: 1

Western result: +--

GST GST-FGA (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: FGA
Full name: fibrinogen, alpha polypeptide; fibrinogen A alpha polypeptide
UniGene Number: N.A.
Accession Number: NP_034326
Source organism: mouse ( Mus musculus )
Full length size (aa): 557
NCBI information page: click here

Full length sequence:
MLSLRVTCLILSVASTVWTTDTEDKGEFLSEGGGVRGPRVVERHQSQCKD
SDWPFCSDDDWNHKCPSGCRMKGLIDEANQDFTNRINKLKNSLFDFQRNN
KDSNSLTRNIMEYLRGDFANANNFDNTYGQVSEDLRRRIEILRRKVIEKA
QQIQALQSNVRAQLIDMKRLEVDIDIKIRSCKGSCSRAVNREINLQDYEG
HQKQLQQVIAKELLPTKDRQYLPALKMSPVPDLVPGSFKSQLQEAPPEWK
ALTEMRQMRMELERPGKDGGSRGDSPGDSRGDSRGDFATRGPGSKAENPT
NPGPGGSGYWRPGNSGSGSDGNRNPGTTGSDGTGDWGTGSPRPGSDSGNF
RPANPNWGVFSEFGDSSSPATRKEYHTGKAVTSKGDKELLIGKEKVTSSG
TSTTHRSCSKTITKTVTGPDGRREVVKEVITSDDGSDCGDATELDISHSF
SGSLDELSERHPDLSGFFDNHFGLISPNFKEFGSKTHSDSDILTNIEDPS
SHVPEFSSSSKTSTVKKQVTKTYKMADEAGSEAHREGETRNTKRGRARAR
PTRGIDT