mouse ( Mus musculus )
Dhh

Western result: +

control Dhh-pep (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: Dhh-pep
CBI Antibody Core Code: A0556
"Lot" Number: 2 Position: 20(143-162)
% Identical Homology of Target to Mouse Protein: 100% (NP_031883.1| desert hedgehog)
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
EGRALDITTSDRDRNKYGLL

Sequence Name: Dhh
Full name: desert hedgehog
UniGene Number: Mm.70066
Accession Number: NM_007857
Source organism: mouse ( Mus musculus )
Full length size (aa): 396
NCBI information page: click here

Full length sequence:
MALPASLLPLCCLALLALSAQSCGPGRGPVGRRRYVRKQLVPLLYKQFVP
SMPERTLGASGPAEGRVTRGSERFRDLVPNYNPDIIFKDEENSGADRLMT
ERCKERVNALAIAVMNMWPGVRLRVTEGWDEDGHHAQDSLHYEGRALDIT
TSDRDRNKYGLLARLAVEAGFDWVYYESRNHIHVSVKADNSLAVRAGGCF
PGNATVRLRSGERKGLRELHRGDWVLAADAAGRVVPTPVLLFLDRDLQRR
ASFVAVETERPPRKLLLTPWHLVFAARGPAPAPGDFAPVFARRLRAGDSV
LAPGGDALQPARVARVAREEAVGVFAPLTAHGTLLVNDVLASCYAVLESH
QWAHRAFAPLRLLHALGALLPGGAVQPTGMHWYSRLLYRLAEELMG