mouse ( Mus musculus )
Bry

Antibody Name/Abbreviation: Bry
CBI Antibody Core Code: A0759
"Lot" Number: 3 Position: 100 (253-352)
% Identical Homology of Target to Mouse Protein: 100% (NP_033335.1| brachyury; brachyury-like 3; brachyury-like 2; low ratio)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
TLCPPASSHPQFGGSLSLPSTHGCERYPALRNHRSSPYPS
PYAHRNSSPTYADNSSACLSMLQSHDNWSSLGVPGHTSML
PVSHNASPPTGSSQYPSLWS

Other western:
Code: A0759
Lot: 2

Western result: +/-

GST GST-Bry (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: Bry
Full name: Brachyury
UniGene Number: N.A.
Accession Number: NM_009309
Source organism: mouse ( Mus musculus )
Full length size (aa): 436
NCBI information page: click here

Full length sequence:
MSSPGTESAGKSLQYRVDHLLSAVESELQAGSEKGDPTERELRVGLEESE
LWLRFKELTNEMIVTKNGRRMFPVLKVNVSGLDPNAMYSFLLDFVTADNH
RWKYVNGEWVPGGKPEPQAPSCVYIHPDSPNFGAHWMKAPVSFSKVKLTN
KLNGGGQIMLNSLHKYEPRIHIVRVGGPQRMITSHCFPETQFIAVTAYQN
EEITALKIKYNPFAKAFLDAKERNDHKDVMEEPGDCQQPGYSQWGWLVPG
AGTLCPPASSHPQFGGSLSLPSTHGCERYPALRNHRSSPYPSPYAHRNSS
PTYADNSSACLSMLQSHDNWSSLGVPGHTSMLPVSHNASPPTGSSQYPSL
WSVSNGTITPGSQTAGVSNGLGAQFFRGSPAHYTPLTHTVSAATSSSSGS
PMYEGAATVTDISDSQYDTAQSLLIASWTPVSPPSM