mouse ( Mus musculus )
mB-5-tubulin

Western result: ++

GST GST-B-5-tubulin (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: B-5-tubulin
CBI Antibody Core Code: A0259
"Lot" Number: 1 Position: 100(1-100)
% Identical Homology of Target to Mouse Protein: 100% (NP_035785.1| tubulin, beta 5)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDS
DLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGP
FGQIFRPDNFVFGQSGAGNN

Sequence Name: mB-5-tubulin
Full name: beta 5 tubulin
UniGene Number: N.A.
Accession Number: NP_035785
Source organism: mouse ( Mus musculus )
Full length size (aa): 444
NCBI information page: click here

Full length sequence:
MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVY
YNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNN
WAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTL
LISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETY
CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADL
RKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMM
AACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK
TAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTG
EGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEEDFGEEAEEEA