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mouse ( Mus musculus )
ATP synthase C mature



Western result: +
control
ATP synthase C mature
(fragment)

32.2 kDa (recombinant)
The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: ATP synthase C mature
CBI Antibody Core Code: A1191

"Lot" Number: 1 Position: 20 (1-20)
% Identical Homology of Target to Mouse Protein: 100% (NP_031532.2| ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c, isoform 1)
Molecular Weight of GST-fusion: 32.2 KDa

Target sequence used to make this antibody:
CSSSPLQVARREFQTSVISR






Sequence Name: ATP synthase C mature
Full name: ATP synthase C mature
UniGene Number: N.A.
Accession Number: NP_031532
Source organism: mouse ( Mus musculus )
Full length size (aa): 95
NCBI information page:

Full length sequence:
CSSSPLQVARREFQTSVISRDIDTAAKFIGAGAATVGVAGSGAGIGTVFG
SLIIGYARNPSLKQQLFSYAILGFALSEAMGLFCLMVAFLILFAM





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© Monday, February 16, 2009 by the CBI Antibody Core and the Center for Biomedical Inventions, University of Texas Southwestern Medical School.