mouse ( Mus musculus )
Remmert mouse gene

Western result: +

GST GST-mCA A1033a (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: mCA
CBI Antibody Core Code: A1033a
"Lot" Number: 1 Position: 100 (1199-1298)
% Identical Homology of Target to Mouse Protein: 73% (XP_488514.1| hypothetical protein XP_488514)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
PVSCNTERLEGGATVPKLQPGLPEARFGSGTPEKNAKAEP
RVDGGCRSRSSSSMPTSPKPLLQSPKPSPSARPSIPQKPR
TASRPEDTPDSPSGPSSPKV

Antibody Name/Abbreviation: mCA
CBI Antibody Core Code: A1033b
"Lot" Number: 1 Position: 20 (1340-1359)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of GST-fusion: 32.2 KDa

Target sequence used to make this antibody:
QEYQEQKQRSSGKDGHQGSK

Sequence Name: Remmert mouse gene
Full name: mouse CARMIL
UniGene Number: N.A.
Accession Number: NP_808475
Source organism: mouse ( Mus musculus )
Full length size (aa): 1374
NCBI information page:

Full length sequence:
MTDESSDVPRELMESIKDVIGRKIKISVKKKVKLEVKGDRVENKVLVLTS
CRAFLLSARIPSKLELTFSYLEIHGVICHKPAQMVVETEKCNMSMKMVSP
EDVSEVLAHIGTCLRRIFPGLSPLKIMKKVSMEPSERLASLQALWDSQTL
AEPGPCGGFSQMYACVCDWLGFSYKEEVQWDVDTIYLTQDTRELNLQDFG
HLEHRDLIPIIAALEYNQWFTKLSSKDLKLSTDVCEQILRVVSRSNRLEE
LVLENAGLRIDFAQKLAGALAHNPNSGLHTINLAGNSLEDRGVSSLSIQF
AQLPKGLKHLNLSKTSLSPKGVNSLCQSLSANPLTASTLTHLDLSGNALR
GDDLSHMYNFLAQPNTIVHLDLSNTECSLEMVCSALLRGCLQCLAVLNLS
RSVFSHRKGKEVPPSFKQFFSSSLALIQINLSGTKLSPEPLKALLLGLAC
NHSLKGVSLDLSNCELGHCLRSGGAQVLEGCIAEIHNITSLDISDNGLES
DLSTLIVWLSKNRSIQHLALGKNFNNMKSKNLTPVLDNLVQMIQDEDSPL
QSLSLADSKLKAEVTIIINALGSNTSLTKVDISGNGMGDMGAKMLAKALQ
INTKLRTVIWDKNNITAQGFQDIAVAMEKNYTLRFMPIPMYDAAQALKTN
PEKTEEALQKIENYLLRNHETRKYLQEQAYRLQQGIVTSTTQQMIDRICV
KVQDHLNSLRACGGDAIQEDLKAAERLMRDAKNSKTLLPNLYHVGGASWA
GASGLSSSPIQETLESMAGEVTRVVDEQLKDWLESMVDAAETLCPSVMRK
AHIRQDLIHASTEKISIPRTFVKNVLLEQSGIDILNKISEVKLTVASFLS
DRIVDEILDSLSSSHRKLANHFSRLNKSLPQREDLEVELVEEKPVKRAIL
TVEDLTEVERLEDLDTCMMTPKSKRKSIHSRMLRPVSRAFEMEFDLDKAL
EEVPIHIEDPPFPSVRQEKRSSGLISELPSEEGRRLEHFTKLRPKRNKKQ
QPTQAAVCTISILPQDGEQNGLMGRVDEGVDEFFTKKVTKMDCKRSSSRS
SDAHELGEGDEKKKRDSRRSGFLNLIKSRSRSERPPTVLMTEELSSPKGA
MRSPPVDTTRKEIKAAEHNGAPDRTEEIKTPEPLEEGPAEEAGRAERSDS
RGSPQGGRRYVQVMGSGLLAEMKAKQERRAACAQKKLGNDVISQDPSSPV
SCNTERLEGGATVPKLQPGLPEARFGSGTPEKNAKAEPRVDGGCRSRSSS
SMPTSPKPLLQSPKPSPSARPSIPQKPRTASRPEDTPDSPSGPSSPKVAL
LPPILKKVPSDKERDGQNSSQSSPRSFSQEASRRSWGPAQEYQEQKQRSS
GKDGHQGSKCSDSGEEAEKEFIFV