mouse ( Mus musculus )
sNHE

Western result: +

control sNHE A1032b (fragment)

32.2 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: sNHE
CBI Antibody Core Code: A1032b
"Lot" Number: 1 Position: 20 (1094-1113)
% Identical Homology of Target to Mouse Protein: 100% (NP_932774.1| sperm-specific sodium proton exchanger)
Molecular Weight of GST-fusion: 32.2 KDa

Target sequence used to make this antibody:
LDETSEEDNGKKENQENEEL

Antibody Name/Abbreviation: sNHE
CBI Antibody Core Code: A1032a
"Lot" Number: 2 Position: 100 (1020-1119)
% Identical Homology of Target to Mouse Protein: 100% (NP_932774.1| sperm-specific sodium proton exchanger)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
IHGSAEDCQLRKIYKAPFLIPVTCHQIQGMEDFTKVMIIQ
TSIAVRKFRWNVRKFIPPRRISMKPDSERESFETLDETSE
EDNGKKENQENEELIEENIN

Sequence Name: sNHE
Full name: sperm sodium/hydrogen exchanger
UniGene Number: N.A.
Accession Number: AY368685
Source organism: mouse ( Mus musculus )
Full length size (aa): 1120
NCBI information page:

Full length sequence:
MHLKDFPIPLPVILFLIGCCFEILSFASTQIQIYADAIQWMDPDIFFGIF
TPVIIFNVAFDMDIYMLQKLFWQILVITIPGFLINYTLILWYLQSVNKLS
LKTVPWLLFSAVLISSDPMLTSASIRDLGLSRSLTNLINGESLLTSVLSL
VIYSGVVHIRFKSKSVNHTLAHKVMSTAWSYIVESFITGIVFTKVIQLWM
ATIFGDDVNHITLIFSVLYLIFYVCELVGMSGIFTLATIGLFLNSTSFKP
GVEAFLLEFWNCLSFIGFLMVFTFIGLLIPAHTYLHISFSDVYYSLNIYF
TLIVLRLLVFLLMSPILSRLGHGFSWRWAFIMVWSEMKGTPNINMALLLA
YSDISLGSERERSQILFHGVSVCVITLIVNRFILPMAVTKLGLRDVTSTK
YKSVYYTFQHFQELTKSTAMALKFDKDLANADWNMVDNAIILQNPYALNQ
EEITEHQKVKCPDCTKEIDETLNIEAMELTNRRLLSAQIASYQRQYRNEV
LSQSAVQVLVGAAGSFGEKKGEYMSPENIKNFSESKKLLSFLRKLLLNWV
YNTKKDKGVPSRYMFLHACHRIVFTNEFEYTGYLVVLMSTYPMIICWISR
LKDIYDNEIKCANYYFLAFYILEALLKVAAMRKEFFSHTWLLFELGITLV
GILDIILIETDSISYNFDLTETVVFMNVIRLLRILRILKLVTPKLLQIID
KRMSQQISFRYSILKGYVQGEMDVLNIIDQIASSKQTKQILLKRVMRNME
HAMKELGYLEYDHPEIAVTMKTKEEINVMLNMAREIVKTFRSKGIIHKVE
GTEINKLIMAKKIQVLDLQSVIQPFNVEEAPCNIPWLSEDPEAITFIQEK
AKVVTFDCGNNIFEEGDEPEGIYVIISGMVKLKRSKPHLEMERVSAESEI
KIHPLPHTEYLLSGEIIGELNCLTKERMQYSATCKTVVETYFIPISHLYE
GFEKRCPNMKHKMWQKIGLAITAQKIREHLSFEDWNYKLQLKLCNAFIRD
IPKSMKTDIYDETVTHVVLIHGSAEDCQLRKIYKAPFLIPVTCHQIQGME
DFTKVMIIQTSIAVRKFRWNVRKFIPPRRISMKPDSERESFETLDETSEE
DNGKKENQENEELIEENINI