fission yeast ( Schizosaccharomyces pombe )
Ago1

Western result: +

GST GST-Ago1 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Ago1
CBI Antibody Core Code: A0970
"Lot" Number: 1 Position: 100 (44-143)
% Identical Homology of Target to Mouse Protein: 34% (XP_486002.1| similar to trinucleotide repeat containing 6; EDIE; GW182 autoantigen)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
VIVGDGSRVPRKQSQLIWNSKEVKQYFGSSWMNSVYDGRS
MCWSKGDIADGTIKVNIGSESHPREIEFSIQKSSKINLHT
LSQFVNSKYSSDPQVLSSIM

Sequence Name: Ago1
Full name: Ago1
UniGene Number: N.A.
Accession Number: N.A.
Source organism: fission yeast ( Schizosaccharomyces pombe )
Full length size (aa): 834
NCBI information page:

Full length sequence:
MSYKPSSEIALRPGYGGLGKQITLKANFFQIISLPNETINQYHVIVGDGS
RVPRKQSQLIWNSKEVKQYFGSSWMNSVYDGRSMCWSKGDIADGTIKVNI
GSESHPREIEFSIQKSSKINLHTLSQFVNSKYSSDPQVLSSIMFLDLLLK
KKPSETLFGFMHSFFTGENGVSLGGGVEAWKGFYQSIRPNQGFMSVNVDI
SSSAFWRNDSLLQILMEYTDCSNVRDLTRFDLKRLSRKFRFLKVTCQHRN
NVGTDLANRVYSIEGFSSKSASDSFFVRRLNGEEQKISVAEYFLENHNVR
LQYPNLPCILVKNGAMLPIEFCFVVKGQRYTAKLNSDQTANMIRFAVQRP
FERVQQIDDFVHQMDWDTDPYLTQYGMKIQKKMLEVPARVLETPSIRYGG
DCIERPVSGRWNLRGKRFLDPPRAPIRSWAVMCFTSTRRLPMRGIENFLQ
TYVQTLTSLGINFVMKKPPVLYADIRGSVEELCITLYKKAEQVGNAPPDY
LFFILDKNSPEPYGSIKRVCNTMLGVPSQCAISKHILQSKPQYCANLGMK
INVKVGGINCSLIPKSNPLGNVPTLILGGDVYHPGVGATGVSIASIVASV
DLNGCKYTAVSRSQPRHQEVIEGMKDIVVYLLQGFRAMTKQQPQRIIYFR
DGTSEGQFLSVINDELSQIKEACHSLSPKYNPKILVCTTQKRHHARFFIK
NKSDGDRNGNPLPGTIIEKHVTHPYQYDFYLISHPSLQGVSVPVHYTVLH
DEIQMPPDQFQTLCYNLCYVYARATSAVSLVPPVYYAHLVSNLARYQDVT
ADDTFVETSEASMDQEVKPLLALSSKLKTKMWYM