fission yeast ( Schizosaccharomyces pombe )
phYH (PAXH)

Western result: +

GST GST-phYH (PAXH) (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: phYH (PAXH)
CBI Antibody Core Code: A0673
"Lot" Number: 1 Position: 100(454-553)
% Identical Homology of Target to Mouse Protein: 31% (XP_143616.4| similar to hypothetical protein FLJ13511)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
VSNNREELPSFRKRSSSSRQSEEIASLQAENAVFSSDPAS
NDEKTGNKKRKKKSVSWKPDNDLVQVKFIESLNEEGAASV
KTPHIYGNARDMDRQEARVA

Sequence Name: phYH (PAXH)
Full name: SPCC74.02c, hypothetical serine rich protein
UniGene Number: N.A.
Accession Number: CAA20832.1
Source organism: fission yeast ( Schizosaccharomyces pombe )
Full length size (aa): 710
NCBI information page: click here

Full length sequence:
MDNWNSVRNVSSDRQTSKTSENPPHTSNEYSGKPEFINLSPDLEENLDEK
LMSAFPGLEPHVFQHSQSPLSHKDASLLATMPSVASSNPSLISSGSSQTG
SPSQSLSSNKEPSSPGISPSNDSQSQNTNHTSISANPYVNNPSHTSRNPD
SGSSLNTASHEVPSSKSDVNVQMLARLKSKSRQKISSSDPLEDLRLTLTE
CLNPINIVQAPKECAAILVNLMSNITQDDQKLVFLDLLKSKVGNSIYSQL
VDGGRKLFLPKLRNWFVSAIRSKHDELIHLILLVLANLPLTTEKLAEVKF
GKPILIVKKKSTNSVIRQLAENLSELAEKSFTIEQNRENEKSSTKNDSTV
SSSAVVMAPAGPAMAPSASNKPSASSTTKSSNSKSKKKVTSISGTSFFKN
LASSTKPTSASSSTKAPLTKQQTNPSTPLSSIMAGLKGREKEKDKDSGIS
SENVSNNREELPSFRKRSSSSRQSEEIASLQAENAVFSSDPASNDEKTGN
KKRKKKSVSWKPDNDLVQVKFIESLNEEGAASVKTPHIYGNARDMDRQEA
RVAFGSHVEDDVENEIIWYKPVPIKFEISKDEIHPRGYKCGGNERNLTPE
ATSEIEREKNESKDISTFNIILDLPVIREFDDSRPPAHIKLVSSDTQATT
ELGFNGLVQQVSENNTNAYSATSNSQLSSIFSNLSSSISDASSNVLQNPS
LSIPNYSNAI