fission yeast ( Schizosaccharomyces pombe )
PCNA

Western result: +++

GST GST-PCNA (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: PCNA
CBI Antibody Core Code: A0182
"Lot" Number: 1 Position: 100(100-199)
% Identical Homology of Target to Mouse Protein: 44% (NP_035175.1| proliferating cell nuclear antigen)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
NLVFESEKNDRISDYDVKLMDIDQEHLGIPDIEYDATITM
PAAEFQRITRDLLTLSDSVTINASKEGVRFSCKGDIGNGS
TTLKQHTDLSDQDQSIEISL

Sequence Name: PCNA
Full name: Proliferating cell nuclear antigen
UniGene Number: N.A.
Accession Number: Q03392
Source organism: fission yeast ( Schizosaccharomyces pombe )
Full length size (aa): 260
NCBI information page: click here

Full length sequence:
MLEARFQQAALLKKLLDAIKELVTDANFDCNDNGISLQAMDSSHVALVSM
LIKSDGFEPYRCDRNIALGINLNALSKVLRCAQNEDLVTLKAEDTPEVLN
LVFESEKNDRISDYDVKLMDIDQEHLGIPDIEYDATITMPAAEFQRITRD
LLTLSDSVTINASKEGVRFSCKGDIGNGSTTLKQHTDLSDQDQSIEISLT
QAVTLTFSLKYLAQFTKATPLATRVTLSMSNDVPLLVEYKMESGFLRFYL
APKIGEEDEE