Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Spt3

Western result: +

GST GST-Spt3 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Spt3
CBI Antibody Core Code: A0966
"Lot" Number: 1 Position: 100 (73-172)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
AKVNRLRTYLSWKDLRKNAKDQDASAGVASGTGNPGAGGE
DDLKKAGGGEKDEKDGGNMMKVKKSQIKLPWELQFMFNEH
PLENNDDNDDMDEDEREANI

Sequence Name: Spt3
Full name: YDR392W
UniGene Number: N.A.
Accession Number: N.A.
Source organism: Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Full length size (aa): 337
NCBI information page:

Full length sequence:
MMDKHKYRVEIQQMMFVSGEINDPPVETTSLIEDIVRGQVIEILLQSNKT
AHLRGSRSILPEDVIFLIRHDKAKVNRLRTYLSWKDLRKNAKDQDASAGV
ASGTGNPGAGGEDDLKKAGGGEKDEKDGGNMMKVKKSQIKLPWELQFMFN
EHPLENNDDNDDMDEDEREANIVTLKRLKMADDRTRNMTKEEYVHWSDCR
QASFTFRKNKRFKDWSGISQLTEGKPHDDVIDILGFLTFEIVCSLTETAL
KIKQREQVLQTQKDKSQQSSQDNTNFEFASSTLHRKKRLFDGPENVINPL
KPRHIEEAWRVLQTIDMRHRALTNFKGGRLSSKPIIM