Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
YGL244W (RTF1)

Western result: +

GST GST-YGL244W (RTF1) (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: YGL244W (RTF1)
CBI Antibody Core Code: A0150
"Lot" Number: 1 Position: 100(44-143)
% Identical Homology of Target to Mouse Protein: 35% (XP_129366.5| similar to centrosome spindle pole associated protein)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
RIEVGSVEDDDEEDDYNPYSVGNADYGSEEEEEANPFPLE
GKYKDESDREHLESLPEMERETLLFERSQIMQKYQERKLF
RARGRDMKEQQQRAKNDEDS

Sequence Name: YGL244W (RTF1)
Full name:
UniGene Number: N.A.
Accession Number: Z72766
Source organism: Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Full length size (aa): 558
NCBI information page: click here

Full length sequence:
MSDLDEDLLALAGADESEEEDQVLTTTSAKRAKNNDQSLSKKRRIEVGSV
EDDDEEDDYNPYSVGNADYGSEEEEEANPFPLEGKYKDESDREHLESLPE
MERETLLFERSQIMQKYQERKLFRARGRDMKEQQQRAKNDEDSRKTRAST
RSTHATGHSDIKASKLSQLKKQRARKNRHYSDNEDEDDEEDYREEDYKDD
EGSEYGDDEEYNPFDRRDTYDKREEVEWAEEEDEQDREPEISDFNKLRIG
RSFVAKFCFYPGFEDAVKGCYGRVNVGTDKRTGKTSYRMVRIERVFLQKP
YNMGKFYTNQYFGVTQGKDRKVFQMNYFSDGLFAEDEYQRYLRALDNSQM
IKPSLHSLSNKTKEVMDFVNTPLTDKTTDEVVRHRMQFNKKLSGTNAVLE
KTVLREKLQYAKETNNEKDIAKYSAQLRNFEKRMSVYEKHHENDQSDIKK
LGELTSKNRKLNMSNIRNAEHVKKEDSNNFDSKSDPFSRLKTRTKVYYQE
IQKEENAKAKEIAQQEKLQEDKDAKDKREKELLVAQFRRLGGLERMVGEL
DIKFDLKF