Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Rpn1

Western result: +

GST GST-Rpn1 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Rpn1
CBI Antibody Core Code: A0028
"Lot" Number: 2 Position: 629-728
% Identical Homology of Target to Mouse Protein: 78% (NP_032977.1| proteasome 26S non-ATPase subunit 4; anti secretory factor 1; multiubiquitin-chain-binding protein)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
VKGEEDADEEETAEGQTNSISDFLGEQVNEPTKNEEAEIE
VDEMEVDAEGEEVEVKAEITEKKNGESLEGEEIKSEEKKG
KSSDKDATTDGKNDDEEEEK

Sequence Name: Rpn1
Full name: YHR027C
UniGene Number: N.A.
Accession Number: NP_011892
Source organism: Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Full length size (aa): To be posted
NCBI information page: click here

Full length sequence:
mvdesdkkqqtideqsqispekqtpnkkdkkkeeeeqlseedaklktdle
llverlkeddsslyeaslnalkesiknstssmtavpkplkflrptypdlc
siydkwtdpnlkssladvlsilamtysengkhdslryrllsdvsdfegwg
heyirhlaleigevyndqvekdaedetssdgsksdgsaatsgfefskedt
lrlcldivpyflkhngeedavdllleiesidklpqfvdentfqrvcqymv
acvpllpppedvaflktaysiylsqneltdaialavrlgeedmirsvfda
tsdpvmhkqlayilaaqktsfeyegvqdiigngklsehflylakelnltg
pkvpediykshldnsksvfssagldsaqqnlassfvngflnlgycndkli
vdndnwvyktkgdgmtsavasigsiyqwnldglqqldkylyvdepevkag
allgigisasgvhdgevepallllqdyvtnpdtkissaailglgiafags
kndevlglllpiaastdlpietaamaslalahvfvgtcngdittsimdnf
lertaielktdwvrflalalgilymgqgeqvddvletisaiehpmtsaie
vlvgscaytgtgdvlliqdllhrltpknvkgeedadeeetaegqtnsisd
flgeqvneptkneeaeievdemevdaegeevevkaeitekkngeslegee
ikseekkgkssdkdattdgknddeeeekeagivdelayavlgialialge
digkemslrhfghlmhygnehirrmvplamgivsvsdpqmkvfdtltrfs
hdadlevsmnsifamglcgagtnnarlaqllrqlasyysreqdalfitrl
aqgllhlgkgtmtmdvfndahvlnkvtlasilttavglvspsfmlkhhql
fymlnagirpkfilalndegepikvnvrvgqavetvgqagrpkkitgwit
qstpvllnhgeraeletdeyisytshiegvvilkknpdyreee