Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
yRAD4

Western result: +/-

GST GST-yRAD4 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: yRAD4
CBI Antibody Core Code: A0310
"Lot" Number: 1 Position: 100(288-387)
% Identical Homology of Target to Mouse Protein: 26% (NP_473443.1| influenza virus NS1A binding protein)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
PPDFTNMKIDTSLNGNNAYKDMVKYPIFWCEVWDKFSKKW
ITVDPVNLKTIEQVRLHSKLAPKGVACCERNMLRYVIAYD
RKYGCRDVTRRYAQWMNSKV

Sequence Name: yRAD4
Full name: ORF YER162C
UniGene Number: N.A.
Accession Number: AAB64689
Source organism: Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Full length size (aa): 754
NCBI information page: click here

Full length sequence:
MNEDLPKEYFELIRKALNEKEAEKAPLSRRRRVRRKNQPLPDAKKKFKTG
LNELPRESVVTVNLDSSDDGVVTVPTDDSVEEIQSSEEDYDSEEFEDVTD
GNEVAGVEDISVEIKPSSKRNSDARRTSRNVCSNEERKRRKYFHMLYLVC
LMVHGFIRNEWINSKRLSRKLSNLVPEKVFELLHPQKDEELPLRSTRKLL
DGLKKCMELWQKHWKITKKYDNEGLYMRTWKEIEMSANNKRKFKTLKRSD
FLRAVSKGHGDPDISVQGFVAMLRACNVNARLIMSCQPPDFTNMKIDTSL
NGNNAYKDMVKYPIFWCEVWDKFSKKWITVDPVNLKTIEQVRLHSKLAPK
GVACCERNMLRYVIAYDRKYGCRDVTRRYAQWMNSKVRKRRITKDDFGEK
WFRKVITALHHRKRTKIDDYEDQYFFQRDESEGIPDSVQDLKNHPYYVLE
QDIKQTQIVKPGCKECGYLKVHGKVGKVLKVYAKRDIADLKSARQWYMNG
RILKTGSRCKKVIKRTVGRPKGEAEEEDERLYSFEDTELYIPPLASASGE
ITKNTFGNIEVFAPTMIPGNCCLVENPVAIKAARFLGVEFAPAVTSFKFE
RGSTVKPVLSGIVVAKWLREAIETAIDGIEFIQEDDNRKEHLLGALESWN
TLLLKLRIRSKLNSTYGKIAEEEPNVTKEQNIADNHDNTETFMGGGFLPG
IANHEARPYSEPSEPEDSLDYVSVDKAEESATDDDVGEDYSDFMKELEMS
EESD