Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Oxa1

Western result: +

GST GST-Oxa1 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Oxa1
CBI Antibody Core Code: A0681
"Lot" Number: 1 Position: 100(147-246)
% Identical Homology of Target to Mouse Protein: 31% (XP_127742.3| oxidase assembly 1-like)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
YVKSSDTVARNSHIKPELDALNNKLMSTTDLQQGQLVAMQ
RKKLLSSHGIKNRWLAAPMLQIPIALGFFNALRHMANYPV
DGFANQGVAWFTDLTQADPY

Sequence Name: Oxa1
Full name: YER154W
UniGene Number: N.A.
Accession Number: NP_011081
Source organism: Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Full length size (aa): 402
NCBI information page: click here

Sequence Notes: Mediates the export of proteins from the mitchondrial matrix to the intermembrane space.; Oxa1p

Full length sequence:
MFKLTSRLVTSRFAASSRLATARTIVLPRPHPSWISFQAKRFNSTGPNAN
DVSEIQTQLPSIDELTSSAPSLSASTSDLIANTTQTVGELSSHIGYLNSI
GLAQTWYWPSDIIQHVLEAVHVYSGLPWWGTIAATTILIRCLMFPLYVKS
SDTVARNSHIKPELDALNNKLMSTTDLQQGQLVAMQRKKLLSSHGIKNRW
LAAPMLQIPIALGFFNALRHMANYPVDGFANQGVAWFTDLTQADPYLGLQ
VITAAVFISFTRLGGETGAQQFSSPMKRLFTILPIISIPATMNLSSAVVL
YFAFNGAFSVLQTMILRNKWVRSKLKITEVAKPRTPIAGASPTENMGIFQ
SLKHNIQKARDQAERRQLMQDNEKKLQESFKEKRQNSKIKIVHKSNFINN
KK