Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Fkh2p

Western result: +

GST GST-Fkh2p (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Fkh2p
CBI Antibody Core Code: A0778
"Lot" Number: 1 Position: 100(611-710)
% Identical Homology of Target to Mouse Protein: 24% (NP_853626.1| protein phosphatase 1, regulatory (inhibitor) subunit 9A; neurabin; neural tissue-specific F-actin binding protein)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
SDSADKSTNNNGGTKMNLPAISTSSLDENGNLEPTTTTSS
GNSNSVPQTGTTTSSLAANSLRLSQPYDTLLRSPTKAFHI
TAMEAYTPERGSANRARSPL

Sequence Name: Fkh2p
Full name: fkh2 or ynl068c or n2403 or ynl2403c
UniGene Number: N.A.
Accession Number: P41813
Source organism: Bakers yeast (Yeast) ( Saccharomyces cerevisiae )
Full length size (aa): 862
NCBI information page: click here

Full length sequence:
MSSSNFNEMNELNMTQTNYGSTKYTAQHHQGVINAIISSLTAPDQPTTVS
LQYSNDKNMATEIQAYAKLSGPNWTYYVKDLEVSIGRNTDPLNSALQENS
DGVKNSYRVNIDLGPAKVVSRKHAIIKYNMNIGGWELHILGRNGAKVNFQ
RTHNGPNNPPIRLSSGTLLDIGGTQMMFILPDSDPVVAPICIEHLMPNLI
NMFGLEGNNNPLLRDIIKQSNYAKQRQLTSNQQIKGFKLYGSGGNAPFGS
GANLGPSEQGIFNNNNNSKNKNGYFTSINPNYTASTTTSNTINPQAASPQ
GPPNTIIAANFVDSYKSSNAYPQALDFTSDLSHDENRNVKPPHSYATMIT
QAILSSPEGVISLADIYKYISSNYAYYRFAKSGWQNSIRHNLSLNKAFEK
VPRRPNEPGKGMKWRISESYQQEFLNKWNTGKVGKIRRGSSVARQLQLHM
AKFNSLPMEMDYRLSLNMAQPPKRQLQSHNVLEPSNNNIIEGFVQHVPSK
GNLPASQQSQPPVSHQNQSQQPPPQEQRQEIQFTFADTQNRNIALARPIK
TPQLQAPNSNANLNQNNMKEYKESLHPPAISISQMNRQSPNNALVSFTNA
CANSKIINNISDSADKSTNNNGGTKMNLPAISTSSLDENGNLEPTTTTSS
GNSNSVPQTGTTTSSLAANSLRLSQPYDTLLRSPTKAFHITAMEAYTPER
GSANRARSPLHSNSNNTNNNGANNSNLQTSGMENKQTGLVLDSNVLKSME
SNNDNRRLTPSTSKSQNVKSSPGVWNLLQFSSTNNTPAADSGGNKRGFSI
NPDIKAKENENATSEKDSDSNSNDLETKDINSSPLKNQGGSTANAKELIL
DTDGAKISIINN